ISSN:
1432-2048
Keywords:
Hypersensitivity
;
Lignification
;
O-methyltransferase
;
Nicotiana (enzyme synthesis during TMV infection)
;
Tobacco mosaic virus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The three tobacco (Nicotiana tabacum L.) S-adenosyl-L-methionine: o-diphenol-O-methyltransferases (OMTs; EC 2.1.1.6) were purified to homogeneity by affinity chromatography on adenosine-agarose. Amounts and catalytic actities of the enzymes were measured in tobacco leaves during the hypersensitive reaction to tobacco mosaic virus. The drastic increase in activity of each enzyme upon infection was shown to arise from the accumulation of enzymatic protein with constant specific enzymatic activity. Rates of OMT synthesis were determined from pulse-labeling experiments with L-[14C]leucine injected into the leaves. The specific radioactivities of the homogenous enzymes were compared in healthy and tobacco mosaic virus-infected tobacco. The results demonstrated that increase in OMT amounts is a consequence of de novo synthesis of the enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392477
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