ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Crystals of the thermoactive recombinant F. pennivorans type I pullulanase, purified from the supernatant of a Bacillus subtilis culture, have been obtained by the vapour-diffusion method in the presence of the inhibitor β-cyclodextrin (2 mM) by mixing protein (15 mg ml−1) with an equal volume of crystallization solution containing 0.1 M bis–tris propane pH 6.5, 50 mM MgCl2 and 15% polyethylene glycol 3350. Crystals diffracted to 3.0 Å using conventional Cu Kα radiation and belong to space group P212121, with unit-cell parameters a = 76.8, b = 96.2, c = 98.5 Å. The asymmetric unit contains one monomer. A preliminary 26% complete data set has been collected at 2.2 Å resolution using synchrotron radiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S090744490001074X
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