ISSN:
1439-0523
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Aegilops variabilis no. 1 is the only known source of resistance to the root-knot nematode Meloidogyne naasi in wheat. Previous studies showed that a dominant gene, Rkn-mn1, was transferred to a wheat translocation line from the donor Ae. variabilis. Random amplified polymorphic DNA (RAPD) analysis was performed on the wheat cultivar ‘Lutin’, on Ae. variabilis, on a resistant disomic addition line and on a resistant translocation line. For genetic and molecular studies, 114-117 BC3F2 plants and F3-derived families were tested. Five DNA and one isozyme marker were linked to Rkn-mn1. Three RAPD markers flanking the Rkn-mn1 locus were mapped at 0 cM (OpY16-1065), 0.8 cM (OpB12-1320) and 1.7 cM (OpN20-1235), respectively. Since the Rkn-mn1 gene remained effective, its introduction into different wheat cultivars by marker-assisted selection is suggested.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1439-0523.2000.00451.x
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