ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Intact histone octamers, produced by a new method quickly and in bulk, were crystallized in KCl/phosphate, and the X-ray data were analysed to 2.15 Å, confirming a P65 space group. This environment preserves the high-resolution structure of the octamers and will be useful for studying them with other functionally important molecules. The octamers form into left-handed superhelices hexagonally spaced by 158.65 Å, having a pitch of 102.57 Å with six octamers per turn. A dipotassium tetraiodo mercurate derivative had good phasing power and should prove valuable in refining the structure after molecular-replacement analysis with lower resolution coordinates; the heavy atom was isomorphously placed at a unique site between the two H3-cysteine residues in the octamer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444999001912
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