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  • 1
    ISSN: 1573-4919
    Keywords: hydroxyl radical ; messenger ; NF-κB activation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Although it is generally believed that reactive oxygen species activate NF-κB, a primary oxidative stress-responsive transcription factor, it is unclear which one among these species causes NF-κB activation. Our hypothesis is that hydroxyl radical (·OH) functions as a messenger for the activation of NF-κB. Jurkat cells, macrophages and JB6 cells were used to test this hypothesis. Cr(VI), silica and ZnO were used as sources of ·OH radicals. None of these ·OH generating systems involves exogenous H2O2. Cr(VI) expressed enhanced activity in induction of NF-κB in Jurkat cells. This activation of NF-κB was decreased by a metal chelator, diethylene triaminepentaacetic acid or a H2O2 scavenger, catalase, but was increased by superoxide dismutase. Mn(II), which reacts with Cr(IV) to inhibit this metal ion-mediated ·OH generation, decreased the NF-κB activation. Sodium formate, an ·OH radical scavenger, also inhibited the NF-κB activation. Electron spin resonance measurements show that Cr(VI) was reduced by Jurket cells to Cr(IV) and Cr(V). During the reduction process, molecular oxygen was reduced to O2- and then to H2O2, which reacted with Cr(IV) and Cr(V) to generate ·OH radical. The ·OH generation correlated with the Cr(VI)-induced NF-κB activation. Similarly, silica caused NF-κB activation in macrophages via the ·OH radical-mediated reaction. This radical was generated via metal mediated reaction from H2O2, which was generated by the reduction of molecular oxygen via O2- as an intermediate during the silica-stimulated ‘respirable burst’. Silica particles did not cause ·OH generation either in Jurket or in JB6 cells and thus did not cause any observable NF-κB activation in these cells. ZnO induced NF-κB activation in JB6 cells through the generation of ·OH resulting from light irradiation of ZnO which was measured by electron spin resonance. The results thus show that ·OH radical functions as a messenger for NF-κB activation. Antioxidants, which scavenge ·OH radical or its precursors, inhibit NF-κB activation. Metal chelators, which make metal ions incapable of generating ·OH from H2O2, inhibit activation of this transcription factor.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4919
    Keywords: aspirin ; antioxidant properties ; silica ; lipid peroxidation ; DNA damage ; NF-κB ; TNF-α
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Electron spin resonance (ESR) was used to investigate the reaction of aspirin toward reactive oxygen species, such as hydroxyl radicals (·OH), superoxide radicals ( O2 - ) and H2O2. The Fenton reaction (Fe(II) + H2O2 ---〉 FE(III) + -OH + OR) was used as a source of -OH radicals. The results show that aspirin is an efficient -OH radical scavenger with a reaction rate constant of k = 3.6 x 1010 M-1sec-1, which is faster than several well established antioxidants, such as ascorbate, glutathione and cysteine. However, aspirin is not a good scavenger for O2 - or H2O2. Through its antioxidant property, aspirin exhibited a protective effect against silica-induced lipid peroxidation and DNA strand breakage. Aspirin also inhibited the activation of nuclear transcription factor-κb induced by silica, lipopolysaccharide or the transition metal, Fe(II), as demonstrated by electrophoretic mobility shift assay. The results show that aspirin functions as an antioxidant via its ability to scavenge -OH radicals. This antioxidant property may explain some of its various physiological and pharmacological actions.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4919
    Keywords: cellular apoptosis ; vanadium ; hydrogen peroxide ; reactive oxygen species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Apoptosis is a physiological mechanism for the control of DNA integrity in mammalian cells. Vanadium induces both DNA damage and apoptosis. It is suggested that vanadium-induced apoptosis serves to eliminate DNA-damaged cells. This study is designed to clarify a role of reactive oxygen species in the mechanism of apoptosis induced by vanadium. We established apoptosis model with murine epidermal JB6 P+ cells in the response to vanadium stimulation. Apoptosis was detected by a cell death ELISA assay and morphological analysis. The result shows that apoptosis induced by vanadate is dose-dependent, reaching its saturation level at a concentration of 100 μM vanadate. Vanadyl (IV) can also induce apoptosis albeit with lesser potency. A role of reactive oxygen species was analyzed by multiple reagents including specific scavengers of different reactive oxygen species. The result shows that vanadate-induced apoptosis is enhanced by NADPH, superoxide dismutase and sodium formate, but was inhibited by catalase and deferoxamine. Cells exposed to vanadium consume more molecular oxygen and at the same time, produce more H2O2 as measured by the change in fluorescence of scopoletin in the presence of horseradish peroxidase. This change in oxygen consumption and H2O2 production is enhanced by NADPH. Taken together, these results show that vanadate induces apoptosis in epidermal cells and H2O2 induced by vanadate plays a major role in this process.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4919
    Keywords: oxygen derived free radicals ; antiooxidants ; cancer ; tea ; epigallocatechin-3-gallate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Electron spin resonance (ESR) spin trapping was utilized to investigate the scavenging effects on hydroxyl radicals (·OH) and superoxide radicals (O2·-) by (-)-epigallocatechin-3-gallate (EGCG), one of the major anticancer compounds in tea. The spin trap used was 5,5-dimethyl-pyrroline N-oxide (DMPO). The Fenton reaction (Fe2+ + H2O2→ Fe3+ +·OH + OH-) was used as a source of ·OH radicals. EGCG efficiently scavenges ·OH radicals with reaction rate of 4.62 × 1011 M- 1sec- 1, which is an order of magnitude higher than several well recognized antioxidants, such as ascorbate, glutathione and cysteine. It also scavenges O2·- radicals as demonstrated by using xanthine and xanthine oxidase system as a source of O2·- radicals. Through its antioxidant properties, EGCG exhibited a protective effect against DNA damage induced by Cr(VI). EGCG also inhibited activation of nuclear transcription factor NF-κB induced by Cr(IV) and 12-o-tetradecanoylphorbol-13-acetate (TPA). The present studies provide a mechanistic basis for the reported anticarcinogenic properties of EGCG and related tea products.
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  • 5
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2016. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 43 (2016): 8936–8944, doi:10.1002/2016GL069785.
    Description: We conducted numerical experiments to simulate elastoplastic deformation of the overriding plate caused by a subducted seamount. Calculations revealed development of a distinct pair of fault-like shear zones, including a landward dipping forethrust fault initiated from the seamount top and a seaward dipping backthrust fault from the landward base of the seamount. Significant dome-shaped surface uplift was predicted above the thrust faults. Lesser-developed seaward dipping backthrust faults were calculated to develop under certain conditions. The overriding plate was calculated to deform in two stages: In Stage I, elastic deformation leads to the formation of fault-like shear zones. After major faults have cut through the entire plate, plastic deformation on faults dominates Stage II. On the subduction interface, compressional normal stress was calculated to increase on the landward leading flank of the seamount and decrease on the seaward trailing flank. These changes, together with associated stress singularities at seamount edges, could affect earthquake processes.
    Description: NSF Grant Number: OCE-1141785; Mariana Trench Initiative of SCSIO; WHOI DOEI Graduate Student Fellowship
    Description: 2017-03-03
    Keywords: Seamount ; Overriding plate ; Elastoplasticity ; Fault formation ; Earthquakes
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
    Publication Date: 2022-05-25
    Description: Author Posting. © The Authors, 2014. This article is posted here by permission of The Royal Astronomical Society for personal use, not for redistribution. The definitive version was published in Geophysical Journal International 197 (2014): 697-704, doi:10.1093/gji/ggu048.
    Description: After the 1960 M9.5 Valdivia, Chile earthquake, three types of geodetic observations were made during four time periods at nearby locations. These post-seismic observations were previously explained by post-seismic afterslip on the downdip extension of the 1960 rupture plane. In this study, we demonstrate that the post-seismic observations can be explained alternatively by volumetric viscoelastic relaxation of the asthenosphere mantle. In searching for the best-fitting viscosity model, we invert for two variables, the thickness of the elastic lithosphere, He, and the effective Maxwell decay time of the asthenosphere mantle, TM, assuming a 100-km-thick asthenosphere mantle. The best solutions to fit the observations in four sequential time periods, 1960–1964, 1960–1968, 1965–1973 and 1980–2010, each yield a similar He value of about 65 km but significantly increasing TM values of 0.7, 6, 10 and 80 yr, respectively. We calculate the corresponding viscoelastic Coulomb stress increase since 1960 on the future rupture plane of the 2010 M8.8 Maule, Chile earthquake. The calculated viscoelastic stress increase on the 2010 rupture plane varies gradually from 13.1 bars at the southern end to 0.1 bars at the northern end. In contrast, the stress increase caused by an afterslip model has a similar spatial distribution but slightly smaller values of 0.1–3.2 bars on the 2010 rupture plane.
    Description: This work was supported by a MIT/WHOI Joint Program Student Fellowship and a Graduate Student Fellowship from the WHOI Deep Ocean Exploration Institute (MD), as well as NSF Grant OCE-1141785 and a Deerbrook Foundation Award (JL).
    Keywords: Seismic cycle ; Transient deformation ; Seismicity and tectonics ; Subduction zone processes ; Dynamics: seismotectonics ; South America
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 7
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    Massachusetts Institute of Technology and Woods Hole Oceanographic Institution
    Publication Date: 2022-05-26
    Description: Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2015
    Description: This thesis investigates lithospheric dynamics of Earth’s subduction zones and Martian tectonic provinces on multiple time scales. In Chapter 2, I use geodetic observations to constrain the post-seismic viscoelastic deformation following the 1960 M9.5 Valdivia, Chile earthquake and quantify its stressing onto the 2010 M8.8 Maule, Chile earthquake. Analysis reveals that the post-1960 viscoelastic process might contribute to the triggering of the 2010 earthquake. Chapter 3 presents numerical experiments to investigate elastoplastic deformation and faulting in the overriding plates of subduction zones caused by the movement of subducted seamounts. Numerical simulations show that normal faults first appear on the seaward side of a subducted seamount, followed by thrust faults on the landward side of the seamount. In Chapter 4, I use the latest Martian gravity and topography data to constrain spatial variations in lithospheric flexure for various tectonic regions on Mars. The effective lithospheric thickness is estimated to be relatively small for the plain regions in southern highlands, but relatively large for the impact basins in northern lowlands as well as for the volcanic montes in the Tharis province. The regional variations in the estimated effective lithospheric thickness might reflect spatial and temporal changes in the thermal state of Mars.
    Keywords: Subduction zones ; Morphotectonics
    Repository Name: Woods Hole Open Access Server
    Type: Thesis
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  • 8
    Publication Date: 2022-10-26
    Description: Author Posting. © American Geophysical Union, 2019. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research-Planets 124, (2019): 3095-3118, doi: 10.1029/2019JE005937.
    Description: We applied localized gravity/topography admittance and correlation analysis, as well as the Markov chain Monte Carlo method, to invert for loading and flexural parameters of 21 subregions on Mars with five distinct tectonic types. The loading styles of the five tectonic types are distinct: The surface and subsurface loading in the polar and plain regions can be assumed to be largely uncorrelated, in contrast to the correlated loading associated with the volcanic montes and Valles Marineris. For the impact basins, we consider the initial topographic depression and mantle plug before postimpact surface loading. Our analyses yield four main results: (1) The inverted effective lithospheric thickness (Te) is highly dependent on assumptions of loading type. (2) There is a trend of increasing Te from the Noachian southern highlands (20–60 km) to the Hesperian northern lowlands (〉90 km) and from the Hesperian Elysium Mons (〈55 km) to the Hesperian/Amazonian Olympus Mons (〉105 km). These Te estimates are consistent with the thermal states at the time of loading, corresponding to a global secular cooling history with decreasing heat flux. (3) Our analyses suggest high‐density basaltic surface loading at the volcanic montes and Isidis basin, in contrast to the low‐density sedimentary surface loading at the Utopia and Argyre basins. (4) We find some degree of correlation between the surface and subsurface loading for the northern polar cap and the northern plains, likely due to earlier, larger polar deposits and ancient buried features, respectively.
    Description: The gravity model JGMRO120d and topography model MarsTopo719 used in this paper were retrieved from the Geosciences Node of NASA's Planetary Data System (http://pds‐geosciences.wustl.edu/mro/mro‐m‐rss‐5‐sdp‐v1/mrors_1xxx/data/shadr/) and from the SHTOOLS package (http://sourceforge.net/projects/shtools/), respectively. The MATLAB codes to reproduce the data analysis, parameter estimation, and key figures are available in a github repository (https://github.com/MinaDing/marslithosphere/tree/v1.0.0, DOI: 10.5281/zenodo.3530057). We are grateful to Mark Wieczorek and Frederik Simons for sharing relevant software online. We thank Ken Tanaka for providing a digital map of Mars chronographic ages. We thank Brandon Johnson for consultation on the loading processes of impact basins. We also thank Editor Laurent Montesi and Steven A. Hauck, as well as Patrick McGovern and anonymous reviewers for their invaluable feedbacks. This work was supported by National Natural Science Foundation of China (41806067, 41890813, 91628301 and U1606401), Key Laboratory of Ocean and Marginal Sea Geology, Chinese Academy of Sciences (OMG18‐02), Chinese Academy of Sciences (Y4SL021001, QYZDY‐SSW‐DQC005 and 133244KYSB20180029), Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0205), Radio Science Gravity investigation of the NASA Mars Reconnaissance Orbiter mission (M.T.Z.), and National Science Foundation (EAR 1220280) and Henry Bigelow Chair for Excellence in Oceanography (J.L.).
    Description: 2020-05-20
    Keywords: Mars ; Lithospheric flexure ; Tectonic loading styles ; Lithospheric strength ; Markov chain Monte Carlo method ; Inverse spectral method
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 9
    Publication Date: 2007-06-01
    Print ISSN: 0006-291X
    Electronic ISSN: 1090-2104
    Topics: Biology , Chemistry and Pharmacology , Physics
    Published by Elsevier
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  • 10
    Publication Date: 2015-06-06
    Description: To elucidate the biological and pathological functions of sialyltransferases (STs), intracellular ST activity evaluation is necessary. Focusing on the lack of noninvasive methods for obtaining the dynamic activity information, this work designs a sensing platform for in situ FRET imaging of intracellular ST activity and tracing of sialylation process. The system uses tetramethylrhodamine isothiocyanate labeled asialofetuin (TRITC-AF) as a ST substrate and fluorescein isothiocyanate labeled 3-aminophenylboronic acid (FITC-APBA) as the chemoselective recognition probe of sialylation product, both of which are encapsulated in a liposome vesicle for cellular delivery. The recognition of FITC-APBA to sialylated TRITC-AF leads to the FRET signal that is analyzed by FRET efficiency images. This strategy has been used to evaluate the correlation of ST activity with malignancy and cell surface sialylation, and the sialylation inhibition activity of inhibitors. This work provides a powerful noninvasive tool for glycan biosynthesis mechanism research, cancer diagnostics and drug development. Scientific Reports 5 doi: 10.1038/srep10947
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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