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  • 1
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    Cultural conditions of arylsulfatase activity in Escherichia coli (2021)
    In:  http://aquaticcommons.org/id/eprint/18548 | 12051 | 2015-11-04 14:56:47 | 18548 | Society of Fisheries Technologists, India
    Details
    Publication Date: 2021-07-10
    Description: Arylsulfatase activity and growth were estimated in Escherichia coli, isolated from marine sediment. Maximum activity was observed at pH 6.6 whereas the maximum growth was at pH 5.6. 2x10ˉ³ M is the optimum substrate concentration for the highest level of enzyme activity/synthesis as well as for its growth. In general higher substrate concentration tended to inhibit enzyme activity and also the growth of the bacterium. Maximum growth and highest enzyme activity occurred at 29°C and above this temperature decreased both of them. Besides these, glucose, sodium sulfate, sodium chloride, sodium dihydrogen phosphate, sodium acetate and ammonium chloride at higher concentrations were inhibiting the enzyme activity and growth. Above 0.2% of glucose, 3% of sodium chloride, 10x10ˉ³ M concentrations of sodium sulfate, sodium dihydrogen phosphate, sodium acetate and ammonium chloride inhibited the activity and growth also. These observations indicate that, to generalize a compound as inhibitor or activator it is difficult since this depends not only on its concentration but also on the source of the enzyme when more than one type is encountered in nature.
    Keywords: Chemistry ; arysulfate sulfohydrolase ; enzymatic activity ; Escherichia coli ; bacteria
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 158-163
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  • 2
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    Inactivation of luminous Vibrio spp. by free chlorine (2021)
    In:  http://aquaticcommons.org/id/eprint/17730 | 9595 | 2015-09-13 09:57:35 | 17730 | Bangladesh Fisheries Research Institute
    Details
    Publication Date: 2021-06-28
    Description: In vitro inactivation of penaeid shrimp larval pathogens, Vibrio iiarveyi and Vsplendidus biovar 1, by free chlorine and the influence of organic matter on thebactericidal activity of chlorine were assessed. More than 5 log unit (〉99.99%) reductionin luminous bacteria from 〉= log 6.00/ml within the first 60 sec of exposure to freechlorine at 1 ppm level was observed. Chlorine was ineffective at 〈50 ppm levels toinhibit luminous Vibrio spp in the presence of 0.1% peptone as interfering organic agent.These results revealed that luminous bacteria are highly susceptible to chlorine but thebactericidal activity of chlorine is affected by organic substance.
    Keywords: Fisheries ; Vibrio spp. ; Chlorine
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 53-58
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  • 3
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    Photosynthetic bacteria in the marine environment at Porto-Novo (2021)
    In:  http://aquaticcommons.org/id/eprint/18452 | 12051 | 2015-10-27 16:01:00 | 18452 | Society of Fisheries Technologists, India
    Details
    Publication Date: 2021-07-09
    Description: Sediment and water samples were collected from mangrove and estuarine biotopes at fortnightly intervals. The physico-chemical characters of the overlying water were studied. In the mangrove biotope maximum temperature (31.5°C) and in the estuarine biotope maximum salinity (35.6‰) were recorded during the summer season, whereas in post-monsoon period the sulphate content was increased to 516 p.p.m. and the pH was reduced to 7.4. Invariably both in the enriched sediment and water samples four major peaks (at wavelengths 460, 705, 772 and 850 nm) and two minor peaks (at wavelengths 580 and 663 nm) of absorption spectra were noticed. A pure culture of Chromatium sp., isolated from mangroves sediment, showed three peaks of absorption spectra at wavelengths, 500, 580 and 850 nm. The effect of sodium chloride on the growth of Chromatium sp., was also studied and it was observed that maximum growth occurred in the range 1-3% sodium chloride concentration. This isolate was also capable of utilizing various sulphur and carbon compounds. Glycerol and glucose did not show any specific effect whereas pyruvate, malate and acetate increased the growth.
    Keywords: Biology ; Ecology ; estuaries ; photosynthesis ; coastal waters ; mangrove swamps ; Chromatium
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 126-130
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  • 4
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    Arylsulfatase activity in marine polychaetes (2021)
    In:  http://aquaticcommons.org/id/eprint/18435 | 12051 | 2015-10-23 18:23:59 | 18435 | Society of Fisheries Technologists, India
    Details
    Publication Date: 2021-07-09
    Description: Marine polychaetes, collected from the Vellar Estuary exhibited arylsulfatase activity. Lumbriconeries sp. Polydora sp. Monojis sp. and Heteromastus sp. were selected for this study. Of these, Heteromastus sp. showed maximum enzymatic activity and it has been chosen for the enzyme kinetic studies such as pH, optimal temperature, period of incubation and the effect of DDT. Enzyme activity showed single peak at pH 6.2 possibly indicating the presence of one type of arylsulfatase. Maximum activity was attained after 12h of incubation at 29°C. DDT has an inhibiting effect on the arylsulfatase activity even at the concentration of 10 p.p.m. and the activity was completely lost at 100 p.p.m.
    Keywords: Chemistry ; marine invertebrates ; enzymes ; Polychaeta ; arylsulphatase
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 57-61
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  • 5
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    Studies on cultural conditions of marine Chromatium buderi Truper and Jannasch 1968 (2021)
    In:  http://aquaticcommons.org/id/eprint/18707 | 12051 | 2015-11-10 22:24:21 | 18707 | Society of Fisheries Technologists, India
    Details
    Publication Date: 2021-07-11
    Description: Photosynthetic characteristics of a purple sulfur bacterium, Chromatium buderi, cultured under different ranges of pH, temperature, light intensities and ammonium chloride concentrations were examined. Maximum bacteriochlorophyll a synthesis was observed at pH 6.5 whereas the optimum growth was at pH 8.0. In general, higher temperature tended to inhibit the chlorophyll a synthesis and growth. 30°C is the optimum temperature both for chlorophyll a synthesis and growth. At 25µE mˉ²Sˉ¹ the bacteriochlorophyll a content and growth attained maximum level. The response to this low light intensity is an adaptation that ensures a high photosynthetic rate for the purple sulfur bacterium that usually occurs in dimly lit environment. Besides these, ammonium chloride at low concentration enhances both chlorophyll a synthesis and growth. Above 0.5% of it causes the nitrogen-chlorosis and also retards the growth of the bacterium. Possible chemical and structural mechanisms involved are discussed.
    Keywords: Aquaculture ; Biology ; photosynthesis ; laboratory culture ; controlled conditions ; pH effects ; environmental effects ; Chromatium buderi ; purple sulfur bacteria
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 87-91
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  • 6
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    Arylsulfatase - producing bacteria in marine sediments (2021)
    In:  http://aquaticcommons.org/id/eprint/18496 | 12051 | 2015-10-30 07:46:45 | 18496 | Society of Fisheries Technologists, India
    Details
    Publication Date: 2021-07-09
    Description: A total of 313 strains of bacteria which hydrolysed tripotassium phenolphthalein disulfate (PDS) were isolated from the sediments of three biotopes, namely, Vellar estuary, backwater and mangrove during the period of investigation. They were identified to the generic level. The following genera were encountered, namely, Vibrio, Bacillus, Alcaligenes, Micrococcus, Pseudomonas, Cytophaga-Flavobacterium, Aeromonas, Corynebacterium and members of Enterobacteriaceae. Vibrio and Bacillus were found to be the dominant groups representing 29.26% and 41.80% respectively of the total isolates. Because of the importance of the Vibrio group in marine environment these isolates were further identified to the species level and it included V. parahaemolyticus, V. alginolyticus, V. consticola, V. anguillarum and V. fischeri. These observations suggest that different groups of arylsulfatase – producing bacteria probably occur in marine sediments.
    Keywords: Biology ; bacteria ; sediments ; enzymes ; Vibrio ; Bacillus ; Alcaligenes ; Micrococcus ; Pseudomonas ; Cytophaga ; Flavobacterium ; Aeromonas ; Corynebacterium ; Enterobacteriaceae
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 121-125
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