ISSN:
1040-7685
Keywords:
regulated isoelectric focusing
;
micropreparative IEF
;
carrier-ampholyte-free IEF
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Three proteins (myoglobin, ribonuclease, and cytochrome C) were concentrated, separated, and isolated from a dilute mixture by electrically controlled focusing in carrier-ampholyte-free solution. Electrolytes were modified at both sides of the capillary. The described method is suitable for the micropreparation of hundred-μg quantities of proteins in times comparable to other capillary techniques (tens of minutes). © 1995 John Wiley & Sons, Inc.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220070305
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