Publication Date:
1989-07-07
Description:
Tomato plants homozygous for the diageotropica (dgt) mutation exhibit morphological and physiological abnormalities which suggest that they are unable to respond to the plant growth hormone auxin (indole-3-acetic acid). The photoaffinity auxin analog [3H]5N3-IAA specifically labels a polypeptide doublet of 40 and 42 kilodaltons in membrane preparations from stems of the parental variety, VFN8, but not from stems of plants containing the dgt mutation. In roots of the mutant plants, however, labeling is indistinguishable from that in VFN8. These data suggest that the two polypeptides are part of a physiologically important auxin receptor system, which is altered in a tissue-specific manner in the mutant.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hicks, G R -- Rayle, D L -- Lomax, T L -- DCB-8718731/DC/NIDCD NIH HHS/ -- New York, N.Y. -- Science. 1989 Jul 7;245:52-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11537490" target="_blank"〉PubMed〈/a〉
Keywords:
Affinity Labels
;
Azides/*metabolism
;
Binding Sites
;
Hypocotyl/cytology/genetics/metabolism/ultrastructure
;
Indoleacetic Acids/*analysis/*metabolism
;
Intracellular Membranes/chemistry/metabolism/ultrastructure
;
Lycopersicon esculentum/cytology/*genetics/metabolism/ultrastructure
;
Microsomes/ultrastructure
;
*Mutation
;
Photolysis
;
*Plant Growth Regulators
;
Plant Proteins
;
Plant Roots/cytology/genetics/metabolism/ultrastructure
;
Receptors, Cell Surface/analysis/genetics/metabolism
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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