Publication Date:
2008-03-07
Description:
Methylation of CpG dinucleotides is generally associated with epigenetic silencing of transcription and is maintained through cellular division. Multiple CpG sequences are rare in mammalian genomes, but frequently occur at the transcriptional start site of active genes, with most clusters of CpGs being hypomethylated. We reported previously that the proximal region of the trefoil factor 1 (TFF1, also known as pS2) and oestrogen receptor alpha (ERalpha) promoters could be partially methylated by treatment with deacetylase inhibitors, suggesting the possibility of dynamic changes in DNA methylation. Here we show that cyclical methylation and demethylation of CpG dinucleotides, with a periodicity of around 100 min, is characteristic for five selected promoters, including the oestrogen (E2)-responsive pS2 gene, in human cells. When the pS2 gene is actively transcribed, DNA methylation occurs after the cyclical occupancy of ERalpha and RNA polymerase II (polII). Moreover, we report conditions that provoke methylation cycling of the pS2 promoter in cell lines in which pS2 expression is quiescent and the proximal promoter is methylated. This coincides with a low-level re-expression of ERalpha and of pS2 transcripts.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kangaspeska, Sara -- Stride, Brenda -- Metivier, Raphael -- Polycarpou-Schwarz, Maria -- Ibberson, David -- Carmouche, Richard Paul -- Benes, Vladimir -- Gannon, Frank -- Reid, George -- England -- Nature. 2008 Mar 6;452(7183):112-5. doi: 10.1038/nature06640.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18322535" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Line, Tumor
;
CpG Islands/genetics
;
DNA/genetics/*metabolism
;
*DNA Methylation/drug effects
;
Doxorubicin/pharmacology
;
Estrogen Receptor alpha/genetics/metabolism
;
*Gene Expression Regulation/drug effects
;
Humans
;
Promoter Regions, Genetic/*genetics
;
RNA Polymerase II/metabolism
;
RNA, Messenger/genetics/metabolism
;
Time Factors
;
Transcription, Genetic/drug effects
;
Tumor Suppressor Proteins/genetics
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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