Publication Date:
1993-07-09
Description:
Transfer RNA (tRNA) splicing is essential in Saccharomyces cerevisiae as well as in humans, and many of its features are the same in both. In yeast, the final step of this process is removal of the 2' phosphate generated at the splice junction during ligation. A nicotinamide adenine dinucleotide (NAD)-dependent phosphotransferase catalyzes removal of the 2' phosphate and produces a small molecule. It is shown here that this small molecule is an NAD derivative: adenosine diphosphate (ADP)-ribose 1"-2" cyclic phosphate. Evidence is also presented that this molecule is produced in Xenopus laevis oocytes as a result of dephosphorylation of ligated tRNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Culver, G M -- McCraith, S M -- Zillmann, M -- Kierzek, R -- Michaud, N -- LaReau, R D -- Turner, D H -- Phizicky, E M -- DE07202-02/DE/NIDCR NIH HHS/ -- GM 22939/GM/NIGMS NIH HHS/ -- GM 25349/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1993 Jul 9;261(5118):206-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8392224" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Diphosphate Ribose/*analogs & derivatives/chemistry/metabolism
;
Animals
;
Cyclic ADP-Ribose
;
Endoribonucleases/metabolism
;
NAD/chemistry/metabolism
;
Oocytes/metabolism
;
Phosphates/metabolism
;
Phosphorylation
;
Phosphotransferases/metabolism
;
*RNA Splicing
;
RNA, Fungal/*metabolism
;
RNA, Transfer/*metabolism
;
Saccharomyces cerevisiae/*genetics
;
Xenopus
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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