Publication Date:
2007-06-16
Description:
Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825053/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825053/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hoskins, Roger A -- Carlson, Joseph W -- Kennedy, Cameron -- Acevedo, David -- Evans-Holm, Martha -- Frise, Erwin -- Wan, Kenneth H -- Park, Soo -- Mendez-Lago, Maria -- Rossi, Fabrizio -- Villasante, Alfredo -- Dimitri, Patrizio -- Karpen, Gary H -- Celniker, Susan E -- P50-HG00750/HG/NHGRI NIH HHS/ -- R01 GM066272/GM/NIGMS NIH HHS/ -- R01 HG000747/HG/NHGRI NIH HHS/ -- R01 HG000747-16/HG/NHGRI NIH HHS/ -- R01 HG00747/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2007 Jun 15;316(5831):1625-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17569867" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chromosome Mapping
;
Chromosomes, Artificial, Bacterial
;
Contig Mapping
;
Drosophila melanogaster/*genetics
;
Genome
;
Heterochromatin/*genetics
;
In Situ Hybridization, Fluorescence
;
Physical Chromosome Mapping
;
*Sequence Analysis, DNA
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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