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  • 1
    Electronic Resource
    Electronic Resource
    THE H-2 HISTOCOMPATIBILITY SYSTEM AND LYMPHOCYTE ADHESION:INTERACTION MODULATION FACTOR INVOLVEMENT (1979)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 6 (1979), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The effects of thymocyte interaction modulation factor on the adhesion of mouse allogeneic thymocytes and B-cells are reported. This glycoprotein, produced by short term cultures of thymocytes, has already been described as reducing the adhesion of syngeneic B-cells, leucocytes and macrophages. Adhesion was measured in suspension culture using the collision efficiency method. This paper reports that:1. In addition to the syngeneic effect of thymocyte IMF in reducing adhesion of certain unlike cell types there is also an allogeneic effect in which an allogeneic T-IMF will diminish the adhesion of a thymocyte population, or still further reduce the adhesion of a B-cell population than would a syngeneic T-IMF.2. Thymocyte IMFs were prepared from different congenic strains and tested on the adhesion of syngeneic and allogeneic thymocytes. When factor and cells were syngeneic or metched at any H-2 locus except H-2 D there was no effect on adhesion since it remained at the same value as in controls in their own IMF. But whenever factor and cells were mismatched at H-2 D there was a marked diminution in the adhesion of the cells.3. Antibodies raised against specific thymocyte IMFs could be used to detect the presence of T-IMF binding to the surface of cells by immunofluorescence or immune cytolysis. These systems show that the antibodies against thymocyte IMF can be used to type the H-2 D type of a cell and that these factors are present at the surface of thymocytes and certain other cell types. They confirm that the thymocyte IMF is either in H-2 D product or is closely associated with H-2 D locus in its binding and action. They also show that the T-IMF antigen on non-lymphocytic types is produced by T-cells or thymocytes.4. The general relevance of these results is discussed in relation to cell recognition phenomena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1979.tb00341.x
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  • 2
    Electronic Resource
    Electronic Resource
    H-2D ANTIGENS RELEASED BY THYMOCYTES AND CELL ADHESION (1981)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 8 (1981), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The identity and complete purification of mouse Thymocyte Interaction Modulation Factor (T IMF) is described. Use of silver-stained PAGE methods shows that previous methods of purification yield preparations containing two protein or glycoprotein bands. T IMF activity from H-2k mice can be bound to 15.5.5 monoclonal antibody columns (anti H-2 Dk) but not to 11.4.1 columns (anti H-2 Kk). The activity can be recovered from 15.5.5 columns and runs on PAGE as a single band at approximately 34,000 Daltons. This evidence together with previous evidence relating the activity to H-2 D locus argues that T IMF is a soluble H-2 D antigen fragment equivalent to a papainized H-2 fragment. Additional evidence is presented on an improved method of assay of T IMF activity, on its inactivation by enzymes and serine-esterase inhibitors and of its effect on syngeneic leucocytes and macrophages. It is also shown that T IMF is not appreciably toxic for cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1981.tb00941.x
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  • 3
    Electronic Resource
    Electronic Resource
    Pattern and Mechanism in the Reaggregation of Sponges (1962)
    [s.l.] : Nature Publishing Group
    Nature 196 (1962), S. 245-248 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] WHEN the various types of cell of an organism reaggregate from a mixture of cells they tend to sort out so that those of each tissue group together. This sorting out demonstrates a specificity which it has been usual to regard as being due to the specific adhesion of cells of like type. The concept ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/196245a0
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  • 4
    Electronic Resource
    Electronic Resource
    A Ribonucleoprotein from Amphibian Gastrulæ (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 185-185 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Estimations of the total nitrogen by the Lubo-chinsky test, phosphorus as phosphate after digestion in concentrated sulphuric acid, ribose by the orcinol reaction, and a-amino nitrogen by the ninhydrin method were made. Deoxyribose and hexose sugars were absent. After hydrolysis in perchloric acid ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/181185a0
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of p H and Temperature on Cell Re-aggregation (1963)
    [s.l.] : Nature Publishing Group
    Nature 200 (1963), S. 1235-1236 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cells from 5-day embryonic chicks and gastrula stages of the amphibian, Xenopus laevis, were used. In examining the effects of temperature chick limb-bud or liver cells were used, being disaggregated by treatment for 5 min with 0.002 M pH. 8.0 solution of ethylenediamine tetra-acetate made up in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2001235b0
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  • 6
    Electronic Resource
    Electronic Resource
    Re-examination of a Supposed Case of Specific Cell Adhesion (1970)
    [s.l.] : Nature Publishing Group
    Nature 226 (1970), S. 260-261 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Another difficulty lies in the possibility that the claimed separation of cells in a mixture of two species types is the result of the aggregation of one species type preceding that of the other, so that aggregates tend to contain cells of one species type alone?a form of behaviour which gives the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/226260a0
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  • 7
    Electronic Resource
    Electronic Resource
    Grooved substrata facilitate in vitro healing of completely divided flexor tendons (1995)
    Springer
    Journal of materials science 6 (1995), S. 266-271 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Multiple grooved substrata with groove depth 5 μm were found to facilitate the healing of completely divided rat flexor tendons in vitro. Sections of tendons cultured on plain substrata showed only partial healing with incompletely sealed epitenon layers and immature thin collagen fibres. Tendons cultured on patterned substrata healed with complete restoration of the epitenon layer and reconstitution of the internal structure of collagen fibres. Epitenon fibroblasts isolated from the surface of rat flexor tendons were shown to be more sensitive to topographical features than fibroblasts of the same size BHK fibroblasts. They remained more elongated and better aligned to the groove direction than BHK cells. Multiple grooved substrata facilitated epitenon cell movement. Cells were found to move with higher speed on patterned substrata than on plain substrata. In summary, we conclude that the use of multiple grooved substrata promotes tendon healing in vitro and may find application in clinical practice in tendon repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00120269
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  • 8
    Electronic Resource
    Electronic Resource
    H–2 restriction of contact inhibition of epithelial cells (1979)
    [s.l.] : Nature Publishing Group
    Nature 281 (1979), S. 222-223 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A number of cell interaction gene loci (CI loci) have been recognised in lymphocyte-lymphocyte and lymphocyte-macrophage interactions5, controlling such reactions as T-B lymphocyte cooperation in antibody responses, and the T-T lymphocyte reactions in the genesis of cytotoxic lymphocytes (CTL)6, as ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/281222a0
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  • 9
    Electronic Resource
    Electronic Resource
    Are there differences in contact inhibition of movement between normal and malignant cells (1974)
    [s.l.] : Nature Publishing Group
    Nature 249 (1974), S. 453-454 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1 Variation of mean overlap ratio with culture age and population density a Age in culture (h) b Population density c Statistical analysis 8 20 24 48 C13 0.183 0.219 0.211 0.298 Py 0.357 0.262 0.255 0.284 〈 70 70-74.9 75-79.9 80-84.9 854 C13 0.207 0.219 0.259 0.212 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/249453a0
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  • 10
    Electronic Resource
    Electronic Resource
    The control of cell division by tension or diffusion (1978)
    [s.l.] : Nature Publishing Group
    Nature 274 (1978), S. 52-53 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We used the technique, described previously6, in which cells are grown on a fabric mesh. They form sheets, 'sails', between the fibres. The sails are composed of confluent, multilayered cells which show division and which are not attached to any substrate, only other cells and the fibres. This ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/274052a0
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