ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Pit-1 is a prototypic member of the POU transcription factor family and plays a critical role in pituitary-specific action of growth hormone (GH), prolactin (PRL), and thyroid-stimulating hormone (TSH) β-subunit genes. The purpose of the present study was to elucidate the changes in the expression of the Pit-1 product in the pituitary of pregnant rats employing an improved double-immunohistochemical method. The positive cells showed nuclear immunoreactivity and Pit-1 protein was frequently observed in the nuclei of many cells which were also immunopositive for GH, PRL, and βTSH. Unexpectedly, a significant number of pituitary cells containing both Pit-1 and gonadotropins were also observed. These cells were usually distributed near blood vessels that supply the pituitary. While a prominent increase in the percentage of Pit-1/PRL, Pit-1/β-luteinizing hormone and Pit-1/β-follicle-stimulating hormone immunoreactive cells was observed in pregnant rats, the percentage of Pit-1/GH immunoreactive cells was strongly decreased. In contrast, no significant differences in the percentage of Pit-1/βTSH doubly immunolabeled cells were noticed. Our findings strongly support the hypothesis that PRL could coexist in the Pit-1 immunopositive gonadotropes. Although Pit-1 protein was not detected in the nuclei of corticotropes, the existence of these cells in the rat pituitary cannot be excluded.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004180050280
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