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  • 1
    Electronic Resource
    Electronic Resource
    Determination of the D and L isomers of some protein amino acids present in soils (1977)
    s.l. : American Chemical Society
    Analytical chemistry 49 (1977), S. 2-7 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac50009a008
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    Paper (German National Licenses)
    Fulltext
  • 2
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    Determination of the D and L isomers of some protein amino acids present in soils (1977)
    American Chemical Society
    Details
    Publication Date: 1977-01-01
    Print ISSN: 0003-2700
    Electronic ISSN: 1520-6882
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society
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    PAPER CURRENT
  • 3
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    Analysis of lipophilic pigments from a phototrophic microbial mat community by high performance liquid chromatography (1988)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: As assay for lipophilic pigments in phototrophic microbial mat communities using reverse phase-high performance liquid chromatography was developed which allows the separation of 15 carotenoids and chloropigments in a single 30 min program. Lipophilic pigments in a laminated mat from a commercial salina near Laguna Guerrero Negro, Baja California Sur, Mexico reflected their source organisms. Myxoxanthophyll, echinenone, canthaxanthin, and zeaxanthin were derived from cyanobacteria; chlorophyll c, and fucoxanthin from diatoms; chlorophyll a from cyanobacteria and diatoms; bacteriochlorophylls a and c, bacteriophaeophytin a, and gamma-carotene from Chloroflexus spp.; and beta-carotene from a variety of phototrophs. Sensitivity of detection was 0.6-6.1 ng for carotenoids and 1.7-12 ng for most chloropigments. This assay represents a significant improvement over previous analyses of lipophilic pigments in microbial mats and promises to have a wider application to other types of phototrophic communities.
    Keywords: Life Sciences (General)
    Type: Journal of microbiological methods (ISSN 0167-7012); Volume 8; 209-17
    Format: text
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    NASA TECHNICAL REPORTS
  • 4
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    Lipophilic pigments from cyanobacterial (blue-green algal) and diatom mats in Hamelin Pool, Shark Bay, Western Australia (1989)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Lipophilic pigments were examined in microbial mat communities dominated by cyanobacteria in the intertidal zone and by diatoms in the subtidal and sublittoral zones of Hamelin Pool, Shark Bay, Western Australia. These microbial mats have evolutionary significance because of their similarity to lithfied stromatolites from the Proterozoic and Early Paleozoic eras. Fucoxanthin, diatoxanthin, diadinoxanthin, beta-carotene, and chlorophylls a and c characterized the diatom mats, whereas cyanobacterial mats contained myxoxanthophyll, zeaxanthin, echinenone, beta-carotene, chlorophyll a and, in some cases, sheath pigment. The presence of bacteriochlorophyll a within the mats suggest a close association of photosynthetic bacteria with diatoms and cyanobacteria. The high carotenoids : chlorophyll a ratios (0.84-2.44 wt/wt) in the diatom mats suggest that carotenoids served a photoprotective function in this high light environment. By contrast, cyanobacterial sheath pigment may have largely supplanted the photoprotective role of carotenoids in the intertidal mats.
    Keywords: Life Sciences (General)
    Type: Journal of phycology (ISSN 0022-3646); Volume 25; 655-61
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    Determination of the D and L isomers of some protein amino acids present in soils (1977)
    In:  Other Sources
    Details
    Publication Date: 2011-08-17
    Description: The D and L isomers of some protein amino acids present in soils were measured by using a gas chromatographic technique. The results of two processing procedures were compared to determine the better method. Results of the comparison indicated that the determination of D and L percentages requires amino acid purification if one is to obtain accurate data. It was found that very significant amounts of D-alanine, D-aspartic acid, and D-glutamic acid were present in the contemporary soils studied. Valine, isoleucine, leucine, proline, and phenylalanine generally contained only a trace to very small amounts of the D isomer. It is probable that the D-amino acids from the alanine, aspartic, and glutamic acids are contributed to the soil primarily via microorganisms. The finding of very significant quantities of some D-amino acids (about 5-16%) in present-day soils may alert some investigators of geological sediments to a possible problem in using amino acid racemization as an age-dating technique.
    Keywords: CHEMISTRY AND MATERIALS (GENERAL)
    Type: Analytical Chemistry; 49; 1, Ja; Jan. 197
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Stereoisomers of isovaline in the Murchison meteorite (1975)
    In:  Other Sources
    Details
    Publication Date: 2011-08-16
    Description: Isovaline is present in the Murchison meteorite as a racemic mixture (about equal concentrations of the R and S enantiomers). Since isovaline does not have a hydrogen atom on its asymmetric alpha-carbon atom, the racemic mixture could not have formed by commonly accepted mechanisms of racemization. Thus, isovaline in the meteorite most probably was synthesized as a racemic mixture and is not the result of the racemization of either the R or S enantiomer. Other chiral amino acids in the meteorite are present as racemic mixtures, and were probably synthesized in a similar manner by abiotic, extraterrestrial processes.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Geochimica et Cosmochimica Acta; 39; Nov. 197
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
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    Circadian rhythm asynchrony in man during hypokinesis. (1972)
    In:  Other Sources
    Details
    Publication Date: 2011-08-16
    Description: Posture and exercise were investigated as synchronizers of certain physiologic rhythms in eight healthy male subjects in a defined environment. Four subjects exercised during bed rest. Body temperature (BT), heart rate, plasma thyroid hormone, and plasma steroid data were obtained from the subjects for a 6-day ambulatory equilibration period before bed rest, 56 days of bed rest, and a 10-day recovery period after bed rest. The results indicate that the mechanism regulating the circadian rhythmicity of the cardiovascular system is rigorously controlled and independent of the endocrine system, while the BT rhythm is more closely aligned to the endocrine system.
    Keywords: BIOSCIENCES
    Type: Journal of Applied Physiology; 33; Nov. 197
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Electron transport in Paracoccus halodenitrificans and the role of Ubiquinone (1984)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: The membrane-bound NADH oxidase of Paracoccus halodenitrificans was inhibited by dicoumarol, 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO), and exposure to ultraviolet light (at 366 nm). When the membranes were extracted with n-pentane, NADH oxidase activity was lost. Partial restoration was achieved by adding the ubiquinone fraction extracted from the membranes. Succinate oxidation was not inhibited by dicoumarol or HQNO but was affected by ultraviolet irradiation or n-pentane extraction. However, the addition of the ubiquinone fraction to the n-pentane-extracted membranes did not restore enzyme activity. These observations suggested the reducing equivalents from succinate entered the respiratory chain on the oxygen side of the HQNO-sensitive site and probably did not proceed through a quinone.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: Canadian Journal of Microbiology (ISSN 0008-4166); 30; 5, 19; 572-577
    Format: text
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    NASA TECHNICAL REPORTS
  • 9
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    Nitrite reduction in paracoccus halodenitrificans: Evidence for the role of a cd-type cytochrome in ammonia formation (1984)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: Cell-free extracts prepared from Paracoccus halodenitrificans catalyzed the reduction of nitrate to ammonia in the presence of dithionite and methyl viologen. Enzyme activity was located in the soluble fraction and was associated with a cytochrome whose spectral properties resembled those of a cd-type cytochrome. Unlike the sissimilatory cd-cytochrome nitrate reductase associated with the membrane fraction of P. halodenitrificans, this soluble cd-cytochrome did not reduce nitrite to nitrous oxide.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-TM-85883 , A-9562 , NAS 1.15:85883
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 10
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    Electron Transport in Paracoccus Halodenitrificans and the Role of Ubiquinone (1983)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: The membrane-bound NADH oxidase of Paracoccus halodenitrificans was inhibited by dicoumarol, 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO), and exposure to ultraviolet light (at 366 nm). When the membranes were extracted with n-pentane, NADH oxidase activity was lost. Partial restoration was achieved by adding the ubiquinone fraction extracted from the membranes. Succinate oxidation was not inhibited by dicoumarol or HQNO but was affected by ultraviolet irradiation or n-pentane extraction. However, the addition of the ubiquinone fraction to the n-pentane-extracted membranes did not restore enzyme activity. These observations suggested the reducing equivalents from succinate entered the respiratory chain on the oxygen side of the HQNO-sensitive site and probably did not proceed through a quinone.
    Keywords: LIFE SCIENCES (GENERAL)
    Type: NASA-TM-84382 , A-9269 , NAS 1.15:84382
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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