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  • 1
    Publication Date: 1994-08-01
    Print ISSN: 0167-4781
    Electronic ISSN: 1879-2634
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Published by Elsevier
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  • 2
    ISSN: 1432-1432
    Keywords: Lysozyme ; Insect ; Lepidoptera ; Evolution ; Sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sequence studies of the N-terminal halves of the lysozymes isolated fromBombyx mori, Galleria mellonella andSpodoptera littoralis (Lepidoptera) allow us to classify these enzymes among the c (chicken) type lysozymes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-8248
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Three NPV isolates ofSpodoptera littoralis Boisduval (Egypt, Maroc and Lyon isolates) are studied and compared to NPV ofBombyx mori L.,Galleria mellonella L.,Porthetria dispar L. andAutographa californica Speyer. Polyhedron protein and peptide analyses as well as immunological comparisons were performed. Each of lepidopterous species possesses a different virus type. The 3 isolates coming fromS. littoralis are similar. Nevertheless, Lyon isolate is a mixture of 2 closely related strains. The 1st strain multiplies inS. littoralis andSpodoptera exigua Hbn. larvae and is found in the 3 isolates. The 2nd strain belongs only to Lyon isolate and does not develop inS. exigua larvae. The polyhedron protein molecular weight of the strain 1 is 30 590 and that of the strain 2 is 28 533. Strain 2 has 1 extrapeptide of MW 80 000 in addition to 11 virion peptides common to the both strains. Attention is drawn to the importance of NPV isolates differences for the bioinsecticide characterization.
    Notes: Résumé Trois isolats de polyèdres nucléaires deSpodoptera littoralis Boisduval (isolats Egypte, Maroc et Lyon) sont comparés entre eux et avec des préparations de polyèdres nucléaires des lépidoptèresBombyx mori L.,Galleria mellonella L.,Lymantria dispar L. etAutographa californica Speyer. L'analyse porte à la fois sur la composition peptidique des virions, sur le poids moléclaire des protéines des polyèdres et sur les parentés antigéniques entre ces protéines. Nous montrons que chaque espèce de lépidoptères possède un type différent de virus et que les trois isolats de polyèdres nucléaires obtenus chezS. littoralis sont très semblables. Cependant l'isolat Lyon diffère des deux autres et se compose d'un mélange de deux souches. La première souche commune aux trois isolats se propage à la fois sur larves deS. littoralis etSpodoptera exigua Hübner, tandis que la seconde souche propre à l'isolat Lyon ne se multiplie que sur larves deS. littoralis.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-8248
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Foreign bodies introduced into lepidopteran larvae induce an increase, often considerable, in amount of lysozyme in the hemolymph. The efficiency of various substances to elicit the release of lysozyme in the larvae varies to a great extent. The effect of Actinomycin D and Cycloheximide introduced in theGalleria mellonella L. larvae stimulated to produce lysozyme by injection of saline or liveMicrococcus luteus was studied. The action of these metabolic inhibitors on the quantity of lysozyme in the hemolymph, 24 h after injection of the inducers, showed that the variations of the amount of the enzyme are under the control of only one mechanism which is not affected by the nature of the inducers. The increase in lysozyme amount of the hemolymph of the inducedG. mellonella larvae is due to the protein synthesis; it does not result from the release of a preformed enzyme as it was demonstrated in other animal species.
    Notes: Résumé L’introduction de corps étrangers dans les larves de lépidoptères entraîne une augmentation parfois considérable du taux de lysozyme dans l’hémolymphe. L’efficacité inductrice de diverses substances varie dans de larges mesures. L’action de l’Actinomycine D et de la Cycloheximide sur la production du lysozyme induit a été recherchée chez des larves deGalleria mellonella L. recevant un stimulus peu spécifique (chlorure de sodium) ou plus spécifique (Micrococcus luteus vivants). L’action de ces inhibiteurs sur la quantité de lysozyme présente dans l’hémolymphe 24 h après induction, indique que les variations du taux de l’enzyme sont sous le contrôle d’un mécanisme unique indépendant de la spécificité des stimuli inducteurs. L’augmentation du taux de lysozyme chez les larves deG. mellonella induites, fait suite à une synthèse protéique et non à la libération d’enzyme préformé.
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