ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
N6-(2-succinyl)adenosine 5′-phosphate and N-(5-amino-1-ribosyl-4-imidazole carbonyl)-L-aspartic acid ′-phosphate were prepared enzymatically using adenylosuccinate lyase (EC 4.3.2.2) isolated from human erythrocytes. N6-(2-succinyl)adenosine 5′-phosphate, N-(5-amino-1-ribosyl-4-imidazole carbonyl)-L-aspartic acid 5′-phosphate and N-(5-amino-1-ribosyl-4-imidazole carboxamide) 5′-phosphate and the nucleoside and free base analogs of these nucleotides were converted to the trimethylsilyl derivatives and characterized by mass spectrometry. The extent of the trimethylsilylation was greater for adenine substituted compounds compared with those related to aminoimidazole carboxamide. Major mass spectral fragment ions correspond to the intact base plus portions of the sugar skeleton and to electron impact-induced decomposition products of the sugar moiety. Succinyl substitution at N6 of the adenine derivatives leads to characteristic loss of CO2 SiMe3 from the molecular ion. A combination of liquid chromatographic procedures and gas chromatography mass spectrometry gave rapid and unequivocal identification of succinyladenine from human urine.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200020205
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