Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
20 (1983), S. 0
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The effects of solution conditions and of formalinisation on the antigenicity of “native” and “pseudo-top” virions of brome mosaic virus (BMV) were studied by double-antibody “sandwich” enzyme-linked immunosorbent assay (DAS-ELISA). Physically characterised BMV morphomers differed serologically from one another and from free coat protein, proving that capsid swelling or lack of RNA cause conformational changes affecting antibody binding. Formaldehyde treatment reduced the antigenicity of virions relative to “native” forms. The results indicate that ELISA detection of viruses is affected both by their physical environment and by chemical treatment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1983.tb00097.x
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