ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Lipopolysaccharides (LPS) from three strains of Bacteroides fragilis were run on SDS-polyacrylamide gels and stained with silver. Each LPS produced a similar pattern, consisting of a series of regularly spaced discrete bands which decreased in intensity as they increased in Mr value. Electroblot transfer from duplicate SDS gels onto nitrocellulose membrane were reacted with antisera raised to whole cells of two of the strains and antigens were visualised with horse-radish peroxidase-antirabbit-IgG conjugate and colour reagent. Results revealed that the two lowest Mr bands of the LPS preparation (rough LPS) represented common antigens.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1983.tb00168.x
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