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  • 1
    Electronic Resource
    Electronic Resource
    Efficient isolation of microspores and the production of microspore-derived embryos from Brassica napus (1987)
    Springer
    Plant cell reports 6 (1987), S. 94-97 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mechanical isolation of Brassica napus microspores from whole buds with a micro-blender enabled the rapid isolation of large numbers of microspores free from tissue and cellular debris. The procedure resulted in savings of time and labor and could be used independent of the size and number of buds to be examined. Between 700 and 1000 embryos per bud were obtained. Compared to anther removal there was no difference in embryo yields or quality. Microspore isolation under cool conditions and overnight incubation prior to plating improved the frequency of embryogenesis. Over 75% of the embryos developed into normal torpedo-stage structures if the medium was replenished during culture and the embryos placed on a gyratory shaker. Over 80% of the torpedo-shaped embryos would ultimately develop into plants. The implications of these techniques to genetic and physiological studies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276661
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  • 2
    Electronic Resource
    Electronic Resource
    The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts (1988)
    Springer
    Plant cell reports 7 (1988), S. 83-87 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Brassica napus L.(cv Topas) plants tolerant to chlorsulfuron (CS) were isolated after selection experiments utilizing microspores and haploid protoplasts. The first microspore-derived plant (M-37,) was CS tolerant, haploid and sterile. Normal plant morphology and fertility was restored after colchicine doubling. A CS tolerant plant was also selected from protoplasts (P-26) isolated from microspore-derived embryo tissue and grown on medium containing CS. P-26 was aneuploid, CS tolerant and had very low fertility. The two selected lines produced selfed progeny which were tolerant to from 10–100 times the CS levels of the corresponding Topas plants. Microspores and protoplasts derived from the selfed plants were also CS tolerant. The segregation pattern for CS tolerance from reciprocally crossed progeny of M-37 and Topas was consistent with a semi-dominant nuclear mode of inheritance. Biochemical analysis of the two mutants indicated that the microspore-derived mutant and F1 crosses contained an altered acetohydroxyacid synthase (AHAS) enzyme, while the AHAS activity of the protoplast mutant was similar to Topas. Selfed seed from the M-37 plants have provided tolerance to CS in both greenhouse and field tests. S1 plants from a second microspore selected mutant (M-42) have tolerated 30 g/ha of CS in greenhouse tests. The two single-celled selection systems are discussed and the microspore selection system highlighted as a new method for in vitro selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270110
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of IAA content Modulators on peroxidase activity and on endogenous IAA during cold pretreatment of maize anthers prior to androgenesis (1998)
    Springer
    Plant growth regulation 26 (1998), S. 123-130 
    Details
    ISSN: 1573-5087
    Keywords: androgenesis ; anther ; cold pretreatment ; endogenous IAA ; peroxidase ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A cold pretreatment is usually applied to induce maize androgenesis. Peroxidase activity, including indole-3-acetic acid (IAA) oxidase activity, and endogenous IAA concentrations were followed during a cold pretreatment (14 days, 7°C) in anthers of two maize genotypes, Seneca 60 and DH5×DH7, respectively with a low or high androgenetic response. The most prominent result was the absence of a detectable IAA oxidase activity in DH5×DH7. Adding effectors of IAA-oxidase activity or IAA transport did not affect significantly the crude peroxidase activity of DH5×DH7 anthers while inducing a clear inhibition of androgenesis at higher concentrations. No strict correlation was found between IAA level and physiological response, the low responding variety having as much IAA as DH5×DH7. However, for DH5×DH7, every treatment that lowered the IAA level after 14 days of cold resulted in a decrease in androgenetic response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006131620264
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