ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A cell line, CY-1, was selected in tyrosine free (tyr−) medium after fusion of mouse erythroleukemia (MEL) cells with mitomycin C-treated rat hepatoma cells. MEL cells do not express the enzyme phenylalanine hydroxylase (PH) and are unable to grow in tyr− medium, whereas the rat hepatoma cells constitutively express PH and are able to grow in tyr− medium. CY-1 cells resemble MEL cells morphologically, karyotypically, and in being inducible for hemoglobin synthesis. In contrast to MEL cells, CY-1 expresses PH and is therefore able to grow in tyr− medium. Using a rat cDNA probe for thePH gene, Southern blot analyses were carried out on DNA isolated from CY-1 and parental cells. CY-1 showed the characteristic mousePH gene pattern but the gene copy number was amplified four− to eightfold compared to parental MEL cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01534676
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