ISSN:
1617-4623
Keywords:
Gluconobacter oxydans
;
Glucose dehydrogenase
;
Point mutation
;
Pyrroloquinoline quinone
;
Substrate specificity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Gluconobacter oxydans contains pyrroloquinoline quinone-dependent glucose dehydrogenase (GDH). Two isogenic G. oxydans strains, P1 and P2, which differ in their substrate specificity with respect to oxidation of sugars have been analysed. P1 can oxidize only d-glucose, whereas P2 is also capable of the oxidation of the disaccharide maltose. To investigate the nature of this maltose-oxidizing property we cloned the gene encoding GDH from P2. Expression of P2 gdh in P1 enables the latter strain to oxidize maltose, indicating that a mutation in the P2 gdh gene is responsible for the change in substrate specificity. This mutation could be ascribed to a 1 by substitution resulting in the replacement of His 787 by Asn.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00272157
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