ISSN:
1573-5001
Keywords:
Water-protein exchange
;
BPTI
;
AlcR
;
Off-resonance ROESY
;
Selective excitation
;
Pulsed field gradients
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary In this communication a new NMR experiment for the safe observation and quantification of water-protein exchange phenomena is presented. It combines a water-selective pulse, offering chemical shift-based separation, and the off-resonance ROESY dynamic filter, which permits the elimination of the unwanted intramolecular dipolar cross relaxation of protein protons. Moreover, pulsed field gradients are used for the suppression of radiation damping and the solvent signal. The straightforward incorporation of this sequence in heteronuclear experiments is demonstrated for the case of the DNA-binding domain of the alcohol regulator protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00228149
Permalink