ISSN:
1040-0397
Keywords:
Testosterone thiosemicarbazone
;
Immunology
;
Adsorptive stripping voltammetry
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The electroanalytical behavior of testosterone thiosemicarbazone (TT) has been investigated by linear sweep and differential pulse voltammetry with and without adsorptive preconcentration on a hanging mercury drop electrode (HMDE). The adsorptive stripping response has been evaluated with respect to preconcentration time and potential, drop size, scan rate, and other variables. Measurement of a selected reduction wave enables the determination of TT in the μg L-1 range, with a detection limit of 100 ng L-1 (10 min preconcentration time). The determination can also be carried out in a urine/aqueous NaClO4 (2/8) medium. The immunological interaction of TT with the testosterone specific antibody can be electrochemically monitored via the decrease in the reduction wave, as described previously. This property is discussed as a tool for the determination of testosterone and its specific antibody by competitive immunoassay with amperometric detection in real matrices such as urine.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140070314
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