Publication Date:
2013-11-15
Description:
Although Cyclosporine A(CsA) is an effective immunosuppressant in the treatment of aplastic anemia(AA), its affects on CD4+CD25+ regulatory T cells(Tregs) from patients with AA still remains controversial. We have first developed a novel mouse model of severe aplastic anemia(SAA) induced by administration with IFN-γ and busulphan. In this study, the SAA model female mice were intragastrically administered with CsA at daily dose of 5 mg/kg for 10 days(the CsA-treated group, n=60), the SAA group(n=60) and the un-treated group (n=60) were as control. All mice selected to set up the SAA model developed the typical clinical and pathological patterns of SAA from day 10 post dosing. Most of them presented bleedings in association with anemia and infections. At day 15 after treatment, a mortality of 35% was found in the SAA group, while a mortality of 20% was found in the CsA-treated group because of impaired liver function and infections. In order to explore the effect of CsA on CD4+CD25+ Tregs from the novel mouse SAA model, the mononuclear cells of the peripheral blood and spleen were subjected to assess the percentage of CD4+CD25+ Tregs and the intracellular Foxp3 expression in CD4+CD25+ Tregs by flow cytometry(FCM). In the SAA group, the FCM analysis showed decreased ratios of CD4+CD25+ Tregs in CD4+ T cells and down-expression of Foxp3 in CD4+CD25+ Tregs compared with the un-treated group(P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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