ISSN:
1573-4927
Keywords:
Whitefly biotypes
;
interbreeding
;
heterozygotes
;
B-type
;
Bemisia tabaci
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Polyacrylamide gel electrophoresis and a kinetic microplate assay were used to detect heterozygotes resulting from a cross between B-type and non B-type strains of the whiteflyBemisia tabaci. Both strains were homozygous for different esterase and acetylcholinesterase (AChE) enzymes, and heterozygotes were produced in one of two crosses between B-type females (diploid) and non B-type males (haploid). In reciprocal crosses, however, no female offspring were produced, indicating that fertilization had not occurred. Despite the identification of individuals heterozygous for the esterase and AChE markers, there was clearly a significant degree of reproductive incompatibility between the two strains. The biochemical assays provided a vital component of this study and the advantages of their use are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00554554
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