Publication Date:
2002-02-09
Description:
Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread gene-silencing information between tissues but not to initiate or maintain an RNAi response. One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for systemic RNAi.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Winston, William M -- Molodowitch, Christina -- Hunter, Craig P -- New York, N.Y. -- Science. 2002 Mar 29;295(5564):2456-9. Epub 2002 Feb 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11834782" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Animals, Genetically Modified
;
Caenorhabditis elegans/embryology/*genetics/metabolism
;
Caenorhabditis elegans Proteins/chemistry/*genetics/*physiology
;
Calmodulin-Binding Proteins/genetics
;
Cytoplasm/metabolism
;
Embryo, Nonmammalian/physiology
;
*Gene Silencing
;
Genes, Helminth
;
Germ Cells/metabolism
;
Green Fluorescent Proteins
;
Intestines/metabolism
;
Luminescent Proteins/genetics
;
Membrane Proteins/chemistry/*genetics/*physiology
;
Molecular Sequence Data
;
Mosaicism
;
Muscle Proteins/genetics
;
Muscles/metabolism
;
Mutation
;
Protein Structure, Tertiary
;
RNA, Double-Stranded/*genetics/metabolism
;
RNA, Helminth/*genetics/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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