Publication Date:
2014-11-08
Description:
The cell tropism of human noroviruses and the development of an in vitro infection model remain elusive. Although susceptibility to individual human norovirus strains correlates with an individual's histo-blood group antigen (HBGA) profile, the biological basis of this restriction is unknown. We demonstrate that human and mouse noroviruses infected B cells in vitro and likely in vivo. Human norovirus infection of B cells required the presence of HBGA-expressing enteric bacteria. Furthermore, mouse norovirus replication was reduced in vivo when the intestinal microbiota was depleted by means of oral antibiotic administration. Thus, we have identified B cells as a cellular target of noroviruses and enteric bacteria as a stimulatory factor for norovirus infection, leading to the development of an in vitro infection model for human noroviruses.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401463/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401463/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jones, Melissa K -- Watanabe, Makiko -- Zhu, Shu -- Graves, Christina L -- Keyes, Lisa R -- Grau, Katrina R -- Gonzalez-Hernandez, Mariam B -- Iovine, Nicole M -- Wobus, Christiane E -- Vinje, Jan -- Tibbetts, Scott A -- Wallet, Shannon M -- Karst, Stephanie M -- R01 AI080611/AI/NIAID NIH HHS/ -- R21 AI103961/AI/NIAID NIH HHS/ -- T90 DE021990/DE/NIDCR NIH HHS/ -- T90 DE021990-02/DE/NIDCR NIH HHS/ -- New York, N.Y. -- Science. 2014 Nov 7;346(6210):755-9. doi: 10.1126/science.1257147.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, College of Medicine, University of Florida, Gainesville, FL, USA. ; Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA. Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. ; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA. ; Department of Medicine, Division of Infectious Diseases, University of Florida, Gainesville, FL, USA. ; Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. ; Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, College of Medicine, University of Florida, Gainesville, FL, USA. skarst@ufl.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25378626" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Anti-Bacterial Agents/pharmacology
;
B-Lymphocytes/immunology/*virology
;
Caliciviridae Infections/*immunology/microbiology/virology
;
Cell Line
;
Enterobacteriaceae/drug effects/*physiology
;
Gastroenteritis/*immunology/microbiology/virology
;
Genome, Viral/genetics/physiology
;
Homeodomain Proteins/genetics
;
Humans
;
Intestines/immunology/*microbiology
;
Mice
;
Mice, Mutant Strains
;
Norovirus/*physiology
;
Peyer's Patches/immunology/virology
;
*Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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