Publication Date:
2008-01-19
Description:
Dietary vitamin A deficiency causes eye disease in 40 million children each year and places 140 to 250 million at risk for health disorders. Many children in sub-Saharan Africa subsist on maize-based diets. Maize displays considerable natural variation for carotenoid composition, including vitamin A precursors alpha-carotene, beta-carotene, and beta-cryptoxanthin. Through association analysis, linkage mapping, expression analysis, and mutagenesis, we show that variation at the lycopene epsilon cyclase (lcyE) locus alters flux down alpha-carotene versus beta-carotene branches of the carotenoid pathway. Four natural lcyE polymorphisms explained 58% of the variation in these two branches and a threefold difference in provitamin A compounds. Selection of favorable lcyE alleles with inexpensive molecular markers will now enable developing-country breeders to more effectively produce maize grain with higher provitamin A levels.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933658/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933658/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harjes, Carlos E -- Rocheford, Torbert R -- Bai, Ling -- Brutnell, Thomas P -- Kandianis, Catherine Bermudez -- Sowinski, Stephen G -- Stapleton, Ann E -- Vallabhaneni, Ratnakar -- Williams, Mark -- Wurtzel, Eleanore T -- Yan, Jianbing -- Buckler, Edward S -- S06-GM08225/GM/NIGMS NIH HHS/ -- SC1 GM081160/GM/NIGMS NIH HHS/ -- SC1 GM081160-01/GM/NIGMS NIH HHS/ -- SC1 GM081160-02/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Jan 18;319(5861):330-3. doi: 10.1126/science.1150255.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18202289" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Breeding
;
Carotenoids/*analysis/metabolism
;
Crosses, Genetic
;
Cryptoxanthins
;
Gene Expression Regulation, Plant
;
*Genetic Variation
;
Haplotypes
;
Intramolecular Lyases/*genetics/metabolism
;
Molecular Sequence Data
;
Mutagenesis
;
Nutritive Value
;
Polymorphism, Genetic
;
Quantitative Trait Loci
;
Xanthophylls/analysis/metabolism
;
Zea mays/chemistry/enzymology/*genetics
;
beta Carotene/analysis/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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