ISSN:
0001-1541
Keywords:
Chemistry
;
Chemical Engineering
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A theoretical model for investigating physical phenomena underlying immune complex formation was developed, based on the statistical mechanical theory of associating fluids that identifies each molecule as a hard sphere with a nested point charge and vector dipole. The interaction between binding molecules (epitope-paratope binding) is represented as a cone truncated by two concentric spheres in which the potential energy is a modified square well with respect to particle separation and a square well with respect to mutual molecular orientation. Equilibrium binding results predicted by the model show good agreement with results obtained experimentally for a model system containing a single antigen and a single monoclonal antibody [bovine serum albumin (BSA) - anti-BSA antibody]. Moreover, values obtained for the system isothermal compressibility and the second virial coefficient by both the model and light scattering experiments also show good agreement with one another.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aic.690410427
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