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  • 1
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray studies of recombinant horseradish peroxidase (1995)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 121-123 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: A non-glycosylated form of horseradish peroxidase c extracted from Escherichia coli inclusion bodies and refolded in the presence of haem and Ca2+ ions has been used to grow protein crystals suitable for X-ray diffraction analysis. The crystals are prisms in the trigonal space group P3112 or P3212 with a = b = 158.9 and c = 114.3 Å, and diffract to 1.9 Å. There are four molecules, each of 34 kDa, in the asymmetric unit. The molecules of the asymmetric unit are related by approximate translational symmetry, resulting in pseudo-centerings. Data to approximately 15 Å can thus be described by a lattice of a′ = b′ = 91.7 Å and c′ = 57.1 Å, α = β = 90° and γ = 120°, including four molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444994008723
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  • 2
    Electronic Resource
    Electronic Resource
    White-light Three Dimensional Microscopy using Multiple-image Storing and Decoding (1971)
    [s.l.] : Nature Publishing Group
    Nature 231 (1971), S. 303-306 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The problem of three dimensional microscopy has received considerable attention. Among the most recent methods, so called deep field microscopy1'2 consists of illuminating the specimen with a thin "sheet" of light, perpendicular to the optical axis of the microscope, and recording the topological ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/231303a0
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  • 3
    Electronic Resource
    Electronic Resource
    Public-sector patents on human DNA (1997)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 388 (1997), S. 709-709 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Sir The increasing emphasis of genomics in therapeutic and diagnostic research and development (R&D) suggests a continuing dominant role for the pharmaceutical industry in patenting human DNA. Here we present new data that challenge this broadly accepted trend by ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/388709a0
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  • 4
    Electronic Resource
    Electronic Resource
    Plant DNA patents in the hands of a few (1999)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 399 (1999), S. 405-406 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SirConsolidation in the agrochemical and seed industry continues to shorten the list of owners of ‘enabling’ intellectual property for plant genetic modification and molecular biology. Six major groups of multinational companies are poised to develop and patent ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/20786
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  • 5
    Electronic Resource
    Electronic Resource
    METABOLIC EFFECTS OF TOPICAL SILVER NITRATE THERAPY IN BURNS COVERING MORE THAN FIFTEEN PERCENT OF THE BODY SURFACE (1968)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 150 (1968), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Notes: Electrolyte and water balance studies have been carried out on 20 patients with total body surface burns ranging from 15 to 85%. All patients were treated with 0.5% aqueous AgNo3 applied directly and continuously to the burn wound. The balance studies showed that the loss of Na through the burn wound was directly related to the square area of burn surface and was constant from day to day. Based on this steady and predictable loss of Na in the uninfected burn, a formula has been derived predicting the daily Na replacement necessary to cover the obligatory loss via the burn wound plus the requirements of normal metabolism. This formula has provided an accurate prediction of daily Na requirements in the treatment of thermal burns of widely varying extent, in patients of widely different ages, as judged by water and electrolyte balance studies.The formula provides a useful guide to the level of Na replacement required. Exact tailoring must be carried out to fit the needs of each patient. The direct monitoring of the accuracy of Na replacement by way of the serum Na level has been found cumbersome but intermittently necessary. In the burn patient in metabolic balance and taking a reasonable diet, the level of urinary excretion of Na has been found to be an accurate prediction of serum Na level. A urinary excretion of 40 mEq of Na per liter or above has uniformly reflected a normal serum Na level. The urinary Na has been used as an indicator of accurate replacement therapy replacing the majority of serum Na determinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1968.tb14719.x
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  • 6
    Electronic Resource
    Electronic Resource
    Extrachromosomal replication of copia -based vectors in cultured Drosophila cells (1983)
    [s.l.] : Nature Publishing Group
    Nature 306 (1983), S. 198-200 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Copia is present at 20-40 copies per haploid genome in flies, and at about 150 copies per cell in Drosophila tissue culture cells3,4. It is also found as extrachromosomal circular molecules the structure of which resembles that of an unintegrated circular retroviral provirus2. The integrated copia ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/306198a0
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  • 7
    Electronic Resource
    Electronic Resource
    Ownership of the human genome (1996)
    [s.l.] : Nature Publishing Group
    Nature 380 (1996), S. 387-388 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE patenting of human DNA sequences is the most controversial aspect of the Human Genome Project (HGP). Academic scientists strive to increase knowledge and to publish their findings. Companies, on the other hand, regard the exclusivity gained through patenting as essential to successful ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/380387a0
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  • 8
    Electronic Resource
    Electronic Resource
    Sialic Acid-deficient α 1 -Acid Glycoprotein produced in Certain Pathological States (1964)
    [s.l.] : Nature Publishing Group
    Nature 204 (1964), S. 75-76 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] a!-Acid glycoprotein was isolated by a two-step procedure from certain blood specimens. First, the sera were fractionated according to a modification of the Cohn method 10 (ref. 6). Second, the resulting fractions VI2 were chromatographed on 'Amberlite I.R〈7-50'7 yielding o^-acid glycoprotein ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/204075a0
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  • 9
    Electronic Resource
    Electronic Resource
    Integration ofDrosophila heat-shock genes transfected into culturedDrosophila melanogaster cells (1984)
    Springer
    Somatic cell and molecular genetics 10 (1984), S. 579-588 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have used DNA-mediated gene transfer to introduce intoDrosophila melanogaster cells DNA sequences for which no selective criteria exist. We have introduced a Drosophila heat-shock locus into culturedDrosophila cells by calcium phosphate cotransfection with the copia vector pCV31gpt and selection for xanthine utilization. We recovered cell lines containing between three and about 50 copies of both pCV31 gpt and pMH10A, a cloned 87A7hsp70 heat-shock locus that encodes a mutant 40,000-dalton heat-shock protein (hsp40). The stable inheritance of the transformed DNAs argues that the input DNAs have integrated into the genome. We show that this is indeed the case for one cell line by cloning back the transfected DNA and detecting the flanking chromocentral sequences by in situ hybridization. Surprisingly, the integrated hsp70 genes are not expressed. This report represents the first example of the cointroduction of DNA sequences intoDrosophila cells by cotransfection with a dominant selectable marker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01535223
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  • 10
    Electronic Resource
    Electronic Resource
    Rescue of aDrosophila temperature-sensitive mutant cell line by DNA transfection (1984)
    Springer
    Somatic cell and molecular genetics 10 (1984), S. 573-577 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract At the nonpermissive temperature, the shibirets gene of Drosophilacauses adult paralysis and cell death during development. We have used DNA-mediated gene transfer with wildtype genomic DNA to rescue the lethal phenotype of shits cultured cells and have isolated cell lines which grow at the nonpermissive temperature of 29° C. By using restriction endonucleases, we confirm that this rescue is DNA sequence dependent. Further, cells cotransfected with wildtype genomic DNA and plasmid DNA contain plasmid sequences when selected for survival at 29° C. This confirms the uptake of DNA by the transformed cells and provides a simple temperature selection technique allowing coselection of genes which cannot themselves be selected for.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01535222
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