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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 33 (2002), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of reproduction was investigated on females of Hungarian strain W, French strain F, and their cross-breed 1X whose ovulation was stimulated with carp pituitary (0.3 mg kg−1and, after 12 h, 2.7 mg kg−1) or Ovopel (one-fifth of a pellet per kg and, after 12 h, one pellet per kg). It was found that in the case of Ovopel treatment, the percentage of spawning females of strain F and the cross-breed 1X was higher than in the hypophysed fish compared. The applied ovulation stimulators did not significantly affect the weight of obtained eggs, whereas the significant (P ≤ 0.01) effect was recorded with respect to the quality of eggs after 12-, 24-, 36- and 48-h of incubation. After Ovopel stimulation, the quality of eggs was better. The origin of the females had no statistically significant effect on the weight of eggs although the yield of eggs from fish of strain W was much smaller than that from females of strain F and the 1X cross-breed. The interaction between the ovulation stimulator and the provenance of the females was significant (P ≤ 0.05) for the percentage of live embryos after 48-h of incubation of eggs. Eggs of the best quality (and highest weight) were obtained from fish of strain F and cross-breed 1X treated with Ovopel. In females of strain F that spawned within 6 and 10 h after the second Ovopel injection, the effect of the ovulation time on the weight of eggs was non-significant. It was significant with respect to the percentage of egg fertilization and of live embryos after 36-h of incubation (P ≤ 0.01 and P ≤ 0.05 respectively). The better quality of eggs (and their higher weight) was recorded when this time was shorter.
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  • 2
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects on carp reproduction were investigated in females of the Dor-70 strain and a cross-breed of this line with a Hungarian one of carp pituitary and Ovopel as ovulation stimulators. It was clear that, in the case of the Ovopel treatment, a higher percentage of females spawned, although the weight of eggs obtained was less than that yielded by hypophysed fish, the differences being highly significant (P ≤ 0.01) with respect to the weight in grams and to percentage of female body weight. No statistically significant differences were determined in the quality of eggs, measured by both the fertilization percentage and the percentage of living embryos, obtained after the two stimulators were used. Neither the provenance of females nor the interaction of female provenance and the stimulator has any effect on the traits investigated. The correlation between the percentage of fertilization and the percentage of live embryos was 0.54 for strain Dor-70 and 0.95 for the cross-breed. The multiple regression equations were calculated for strain Dor-70 and for cross-breed 5 separately, where the percentage of living embryos constituted a dependent variable and the mass of a female, mass of eggs in grams and the percentage of fertilization were independent variables. We appraised the regressions as significant for Dor-70 (P ≤ 0.04) and highly significant for the cross-breed (P ≤ 0.0001).
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  • 3
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ovulation was stimulated in carp females of Polish and Hungarian provenance using injections of ( d-Ala6) GnRH ProNHEt (Kobarelin) (20 μg kg−1) with metoclopramide (10 mg kg−1) or carp pituitary (3 mg kg−1). For estimating the effects of the substance stimulating ovulation and of the female provenance, analysis of variance was carried out using the least squares method. It was found that the stimulating substance, provenance and interaction of these factors did not significantly affect the weight of eggs obtained. On the other hand, the stimulating substance, provenance and interaction had a highly significant (P ⩽ 0.0001) effect on the percentages of fertilization and live embryos. Females of Hungarian provenance treated with Kobarelin yielded eggs of much better quality but a smaller weight than after the pituitary treatment. In the case of carp females of Polish provenance, a yield of eggs of smaller weight and very poor quality was noted compared with the weight and quality of eggs obtained after hypophysation.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 31 (2000), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Milt of grass carp Ctenopharyngodon idella (Val.) and goldfish Carassius auratus auratus (L.) was cryopreserved in experiments following &Hf;1 2 and &Hf;1 4 fractional factorial designs. The efficiency of a graphical method and manova was tested, as well as the informative strength of both designs. Fractional factorial design was very suitable for screening experiments, and it allowed separation of important and unimportant effects. A graphical method showed the same efficiency and reliability as manova. The &Hf;1 4 fractional factorial design was less informative than the &Hf;1 2 fractional factorial design, because it did not allow an estimation of the interactions. The application of fractional factorial designs for multifactorial screening experiments on cryopreservation of fish sperm has been implemented in this study.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 34 (2003), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Stimulation of ovulation was carried out in female carp (Cyprinus carpio L.) of Polish line 3, with carp pituitary homogenate, Ovopel (the preparation contains d-Ala6, Pro9NEt-mGnRH and metoclopramide), and Ovopel and carp pituitary homogenate. The effect of the ovulation stimulators on the weight of eggs expressed in grams and as a percentage of female body weight was determined. The effect of the treatments on the quality of eggs expressed as the percentage of living embryos after 24- and 36-h incubation was also determined. The best results were obtained after stimulation with Ovopel (the priming dose) and pituitary homogenate (the resolving dose). In this case all the females spawned, and the highest yield and quality of eggs were recorded. The poorest results were noted if the ovulation was stimulated with repeated injection of carp pituitary homogenate. The females treated with carp pituitary homogenate gave eggs of poorer quality than those stimulated with two doses of Ovopel or with Ovopel and pituitary homogenate. The percentage of living embryos after 24- and 36-h incubation of eggs was significantly higher for the group stimulated with Ovopel and pituitary homogenate than for the other treatments.
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  • 6
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ovulation was stimulated in females of grass carp Ctenopharyngodon idella (Valenciennes) and silver carp Hypophthalmichthys molitrix (Valenciennes) using LHRH-a with pimozide at doses of 15 μg kg− 1 and 5 mg kg− 1 body weight respectively. The results of reproduction after the LHRH-a stimulation were compared with the results of spawning recorded in the case of hypophysation. In the case of grass carp treated with LHRH-a, the percentage of stripped females was higher than that recorded after hypophysation, while the female of silver carp yielded eggs irrespective of the type of applied ovulation stimulator. The average weight of eggs (in grams and as a percentage of female body weight) obtained from both grass carp and silver carp did not differ significantly in the groups treated with LHRH-a and carp pituitary. Average percentages of fertilization and of live embryos did not differ significantly in the groups treated with the two ovulation stimulators. This statement concerns the two species investigated. Statistically significant differences (P≤  0.05) were calculated with respect to the mean percentage of deformed larvae. This percentage was distinctly higher after an LHRH-a treatment in both grass carp and silver carp. Irrespective of the applied ovulation stimulator, a greater weight of eggs of better quality was obtained from silver carp females.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 30 (1999), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ovulation was stimulated in four groups of European catfish, Silurus glanis L., using injections of des-Gly10, [D-Ala6]-LHRH Ethylamide (20 μg kg–1) and pimozide (10 mg kg–1), Ovaprim (0.33 mL kg–1), and carp pituitary extract (4 mg kg–1, in one or two doses). A higher percentage of ovulating females (producing eggs of sufficient quality) was found after the LHRH-a and Ovaprim treatments (100% and 80%) in relation to fish treated with the pituitary extract (60% and 66.67%). The greatest weight of eggs was obtained in the case of repeated hypophysation and LHRH-a (1299.69 and 1298.57 g, respectively), and the smallest after single hypophysation (1144.08 g). After 60 h of incubation, the best quality of eggs was found in the group treated with Ovaprim (62.9% of live embryos) and the poorest in the two groups which underwent hypophysation (50.41% and 50.75%). No statistically significant effect by the ovulation stimulators on the characteristic qualitative and quantitative traits of obtained eggs was ascertained.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Milt of common carp, Cyprinus carpio L. was cryopreserved in pellet form with the use of 12 extenders. Most efficient were: BE2 original extender (containing 85 mM NaCl, 50 mM KCl, 3 mm CaCl2, 1 mm MgCl2 with 10% dimethyl-acetamide (DMA) and 10% addition of hen's egg yolk) and Kurokura et al.'s extender with 15% DMA and 10% yolk (about 73% and 69% of eyed eggs, about 61% and 52% of swim-up larvae, respectively). Within the most effective treatments, survival from the eyed-egg stage to the swim-up stage was similar to that observed in the control group. Survival from the eyed-egg stage to the swim-up stage (percentage of eyed eggs was considered as 100%) was highly significantly and positively correlated with the actual rate of swim-up larvae.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 32 (2001), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of spawning on European catfish females with an average body weight of 5.6 kg and 11.2 kg was investigated, with carp pituitary and Ovopel being used as ovulation stimulators. Ovopel induced ovulation in a higher percentage of females of both smaller and larger body weight. The applied ovulation stimulators did not significantly affect the weight of the obtained eggs. On the other hand, the effect of the stimulators was highly significant (P≤ 0.01) with respect to the percentage of live embryos after 24-, 48- and 56-h incubation. The fish of greater body weight yielded eggs of a significantly (P≤ 0.05) greater weight, although of poorer quality. The interaction between the ovulation stimulator and female body weight was statistically significant (P≤ 0.05) for the weight of the obtained eggs and highly significant (P≤ 0.01) for the percentage of live embryos after 48-h incubation. For the percentage of live embryos after 24- and 56-h incubation, the statistical significance (P≤ 0.05) of this interaction was determined. In the case of females of smaller body weight, a statistically significant (P≤ 0.05) phenotypic correlation was found between the percentage of live embryos after 24-h incubation and that after 48-h incubation, and also between the percentage of live embryos after 24- and 56-h incubation.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 32 (2001), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ovulation stimulation was carried out in carp (Cyprinus carpio L.) females of Lithuanian line B, using carp pituitary (0.3 + 2.7 mg kg−1), Aquaspawn (0.5 mL kg−1) and Aquaspawn + carp pituitary (0.3 mL kg−1 + 2.7 mg kg−1). The poorest results of reproduction (the lowest percentage of ovulating females, the lowest weight of eggs and their poorest quality) were obtained after double hypophysation. The best results were induced by the application of Aquaspawn alone. The effect of the applied stimulators was statistically non-significant with respect to the weight of eggs, being highly significant (P ≤ 0.01) with respect to the percentage of fertilized eggs and that of living embryos after 24- and 36-h incubation. The multiple-regression equations were calculated for the three groups separately. The percentage of living embryos after 36-h incubation constituted a dependent variable while the mass of females, the mass of eggs, the fertilization percentage and the percentage of living embryos after 24-h incubation were independent variables. The regression was only significant (P ≤ 0.01) for the group of fish treated with Aquaspawn.
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