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  • 1
    Electronic Resource
    Electronic Resource
    Influence of metal complexation on the metabolism of citrate byKlebsiella oxytoca (1994)
    Springer
    BioMetals 7 (1994), S. 163-169 
    Details
    ISSN: 1572-8773
    Keywords: biodegradation ; Klebsiella oxytoca ; metal-citrate complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The uptake of 14C-labeled cadmium-, copper and zinc-citrate into cells of Klebsiella oxytoca was followed. The study was made in order to examine if the earlier reported disability of the bacterium to degrade these complexes was due to an inhibition in transport through the cell membrane. Citrate complexed to cadmium, copper or zinc was taken up at a similar rate to the free citric acid. However, the metal-citrate complexes were not metabolized as shown by the marked accumulation of 14C in the cells as compared with the 14C content in the cells incubated with free citric acid. This was confirmed by the results from trichloroacetic acid-precipitation showing that no 14C was incorporated into macromolecules when the citrate was complexed to the different metals. It is suggested that the inhibited degradation was due to effects on the interaction between enzyme (aconitase) and substrate in the conversion of citrate to iso-citrate. The role of complex configuration on the mineralization of metal-citrate is discussed and also tested in mineralization studies of other metal-citrate complexes (aluminum-, calcium-, cobalt-, manganese- and nickel-citrate).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00140487
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    EFFECTS OF METALS ON THE MICROBIAL MINERALIZATION OF ORGANIC ACIDS (1997)
    Springer
    Water, air & soil pollution 94 (1997), S. 45-57 
    Details
    ISSN: 1573-2932
    Keywords: organic acids ; metals ; mineralization ; metal-organiccomplexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The effects of chemical speciation on mineralization of organic compounds was studied using citric acid as a model substance. The degradation of 14C-labeled Al-, Co-, Cu-, and Zn-citrate was followed in chemically well-defined media inoculated with mixed cultures of microorganisms (soil extracts from two soils). The degradation of citrate was completely inhibited when the acid was bound to Zn, Cu, or Co and partly inhibited when bound to Al. The mineralization of citrate as well as histidine was also followed by incubation of the complexes (Cu, Zn, Al) in the two soils. No effect of metals on the degradation of histidine was seen. The degradation of citrate in soils was also unaffected when complexed to Cu and Zn, whereas Al exerted an inhibited decomposition in both soils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026423202978
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of metals on the microbial mineralization of organic acids (1997)
    Springer
    Water, air & soil pollution 94 (1997), S. 45-57 
    Details
    ISSN: 1573-2932
    Keywords: organic acids ; metals ; mineralization ; metal-organic complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The effects of chemical speciation on mineralization of organic compounds was studied using citric acid as a model substance. The degradation of14C-labeled Al-, Co-, Cu-, and Zn-citrate was followed in chemically well-defined media inoculated with mixed cultures of microorganisms (soil extracts from two soils). The degradation of citrate was completely inhibited when the acid was bound to Zn, Cu, or Co and partly inhibited when bound to Al. The mineralization of citrate as well as histidine was also followed by incubation of the complexes (Cu, Zn, Al) in the two soils. No effect of metals on the degradation of histidine was seen. The degradation of citrate in soils was also unaffected when complexed to Cu and Zn, whereas Al exerted an inhibited decomposition in both soils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407092
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  • 4
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    Effects of metals on the microbial mineralization of organic acids (1997)
    Springer
    Details
    Publication Date: 1997-02-01
    Print ISSN: 0049-6979
    Electronic ISSN: 1573-2932
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Springer
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