ISSN:
1432-072X
Keywords:
Rumen bacteria
;
Vanillate
;
Syringate
;
Ferulate
;
Formate
;
Carbohydrates
;
Methanogens
;
Synthrophococcus sucromutans
;
Methanobrevibacter
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Most probable number (MPN) estimates indicated that a mean of 4.3×107 and 5×106 bacteria per ml of rumen fluid from a predominantly alfalfa hay-fed steer demethoxylated ferulate and syringate, respectively. After further enrichment from an MPN tube of the highest dilution showing demethoxylation of syringate, strain S195 was isolated using roll tubes with syringate as an added energy source. S195 was an anaerobic, Gram-negative, nonmotile coccus, 1 to 1.3 μm in diameter, and was unique in using various carbohydrates as electron donor with acetate as the sole organic product. One of the following electron acceptor systems allowed growth (organic products in parentheses): Methanobrevibacter simithii (CH4), formate (acetate), 3,4,5-trimethoxybenzoate and syringate (acetate and gallate), vanillate (acetate and protocatechuate), vanillin (acetate, protocatechuic aldehyde and protocatechuate), ferulate (acetate, caffeate and hydrocaffeate), caffeate (hydrocaffeate). Strain S195 required 30% (v/v) rumen fluid in the medium for good growth. S195 was placed in a new genus and species, Syntrophococcus sucromutans, of the family Veillonellaceae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00412795
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