ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Soluble periplasmic dihaemic cytochrome c4, of 21 293 Da molecular mass, has been characterized from Thiobacillus ferrooxidans, an acidophilic bacteria. The native cytochrome has been purified from the bacteria using ion-exchange chromatography and crystallized using solution 27 of the Hampton Research Crystal Screen II. The crystals belong to the hexagonal space group P6222 or P6422, with unit-cell parameters a = 101.59, b = 101.59, c = 151.59 Å. Frozen crystals diffract to 2.17 Å resolution. The MAD method is currently being used (four Fe atoms per asymmetric unit) to solve the protein structure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900010064
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