ISSN:
1573-4986
Keywords:
monoclonal antibody
;
bacteria receptor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A mouse monoclonal antibody (87.5) against Galα1-4Gal has been obtained after immunization with the disaccharide glycosidically coupled to a protein. The specificity was determined by studying its binding to a number of glycoconjugates and oligosaccharides. The antibody which was found to be highly specific for terminal Galα1-4Gal residues is a powerful tool for the detection of this structure in glycoproteins and glycolipids by immunochemicalin vitro methods. It is also useful forin vitro quantification of the free disaccharide. A thin layer chromatographic overlay assay using glycolipids and an immunoperoxidase technique is also described. The antibody 87.5 is used in this assay to identify human uroepithelium glycolipids with terminal Galα1-4Gal residues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01049374
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