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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Endeavour 11 (1987), S. 221 
    ISSN: 0160-9327
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 2 (1988), S. 0 
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Sludge Treatment and disposal costs contribute a large proportion to the overall operating costs of sewage treatment installations. With the increasing drive towards more cost-effective operation it has been recognized that thickening sludge prior to disposal can achieve significant cost savings for limited capital outlay. This paper describes the practical experiences at the Lundwood, Old Whittington, and Aldwarke works of Yorkshire Water (YW) where digested sludge is gravity thickened in shallow tanks of large surface area prior to land disposal. Existing structures have been utilized where possible to minimize capital cost. Digestion plant operational data for the three works are given, and an evaluation is made of cost savings resulting from the post-digestion thickening process.
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Unstressed leaves of the mutant R5201 were found to contain up to six times the soluble proline of the parent line in the first three leaves over a 16-day period (Table 1). We examined two aspects of pest attack: whether locusts or slugs would choose preferentially to attack and consume mutant ...
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  • 4
    ISSN: 1432-2048
    Keywords: Acetohydroxyacid synthase ; Amino acid (branched-chain) ; Feedback regulation ; Mutant (Nicotiana) ; Nicotiana ; Valine resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acetohydroxyacid synthase (EC 4.1.3.18) has been extracted from leaves of three valine-resistant (Valr) tobacco (Nicotiana tabacum) mutants, and compared with the enzyme from the wild-type. The enzyme from all three mutants is appreciably less sensitive to inhibition by leucine and valine than the wild-type. Two of the mutants, Valr-1 and Valr-6, have very similar enzymes, which under all conditions are inhibited by less than half that found for the wild-type. The other mutant, Valr-7, has an enzyme that only displays appreciably different characteristics from the wild-type at high pyruvate or inhibitor concentrations. Enzyme from Valr-7 also has a higher apparent Km for pyruvate, threefold greater than the value determined for the wild-type and the other mutants. The sulphonylurea herbicides strongly inhibit the enzyme from all the lines, though the concentrations required for half-maximal inhibition of enzyme from Valr-1 and Valr-6 are higher than for Valr-7 or the wildtype. No evidence has been found for multiple isoforms of acetohydroxyacid synthase, and it is suggested that the valine-resistance of these mutant lines is the result of two different mutations affecting a single enzyme, possibly involving different subunits.
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  • 5
    ISSN: 1432-2242
    Keywords: Potato protoplasts ; Tissue culture ; Variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants were obtained from protoplasts of shoot cultures of potato (Solarium tuberosum L. cv. ‘Maris Bard’) and from in situ calluses upon plants of cv. ‘Majestic’. None of the protoplast-derived plants resembled each other in all of ten morphological characteristics scored and only one resembled the parental ‘Maris Bard’ type. As there were a number of plants regenerated from each of ten protoplast-derived calluses it is concluded that variation arose after protoplast isolation during the cell culture phase. Plants regenerated from in situ calluses of cv. ‘Majestic’ were quite uniform. Reported cases of variation and uniformity from cultured potato tissues are discussed. It is concluded that the variation is not a consequence of using protoplasts and that the expression or induction of variation is controllable.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 63 (1982), S. 265-272 
    ISSN: 1432-2242
    Keywords: Potato protoplasts ; Regeneration ; Chromosome variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosomes have been studied in protoplast-derived potato plants of the tetraploid cultivars Maris Bard and Fortyfold. A high degree of aneuploidy was found amongst the regenerants of both cultivars but the nature of the chromosome variation differed. The Maris Bard regenerants were characterised by high chromosome numbers, a wide range of aneuploidy (46–92) and a low percentage of plants with the normal chromosome number (2n = 48), whereas a much higher proportion of the Fortyfold regenerants had 48 chromosomes and the variants were within a more limited aneuploid range. In both cultivars chromosome variation was found between calluses, within calluses and even within shoot cultures. The origin of the chromosome variation and the differences found between the two cultivars are discussed.
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  • 7
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sycamore (Acer pseudoplatanus L.) calluses resistant to azaguanine were isolated after mutagenesis with nitrosoguanidine. One callus SNC61 was highly variable in its growth on 10 μg/ml azaguanine and it was concluded that its resistance was not due to a stable alteration in the callus. Another callus SNE31 was isolated after 4.5 months culture in 0.5 or 1 μg/ml azaguanine which had a 10-fold increase in resistance to azaguanine. This was shown to be accompanied by a reduction to 50% of the normal level of hypoxanthine phosphoribosyltransferase (E.C.2.4.2.8).
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 55 (1979), S. 1-4 
    ISSN: 1432-2242
    Keywords: Selection ; Barley ; Mutant ; S-(2-aminoethyl)L-cysteine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary S-(2-aminoethyl)L-cysteine (AEC) inhibits the growth of mature barley (Hordeum vulgare L vars. ‘Bomi’ and ‘Maris Mink’) embryos grown on sterile medium. This inhibition is relieved by lysine and, to a lesser extent, arginine and ornithine. In order to try and select plants which accumulate lysine, 8200 M2 embryos of sodium azide mutagenised barley were screened for growth in the presence of 0.25 mM AEC. One line, R906 was selected for further characterisation. Progeny of the originally selected plant after selfing were all resistant to AEC. In a reciprocal cross with a sensitive barley the resistant trait was inherited as a single recessive nuclear gene which we designate aec-1.
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  • 9
    ISSN: 1573-5060
    Keywords: Somaclonal variation ; explant culture ; field trials ; potato breeding ; regeneration ; Solanum tuberosum ; potato ; tuber characteristics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tuber characteristics of potato clones (Solanum tuberosum L.) regenerated from cultured leaf, rachis or stem pieces of cv. Desiree were assessed in field trails over three years. In the first two seasons, when the crop was grown for seed potato production, tuber numbers and the tuber yields were normally distributed and the population means of the clones were about the same as those of the controls. When 78 of the clones were grown as a ware crop in a replicated field trial there were statistically significant differences between most clones and the controls in total yield (generally lower) and ware yield (generally higher), brought about by alterations of the size distributions of tubers. In all years the population of clones had fewer scab (Streptomyces scabies) lesions than the controls. Clones with consistent differences in flesh colour and eye depth were also observed. Two out of 78 clones, in 1984, had significantly higher dry matter content. No stable decrease in susceptibility to the cyst nematode Globodera pallida was observed amongst 167 clones tested. These potato plants regenerated from explants clearly show that somaclonal variation can include agronomically relevant changes.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 20 (1982), S. 229-243 
    ISSN: 1573-4927
    Keywords: Hordeum vulgare ; mutant ; aspartate kinase ; isozymes ; amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Barley (Hordeum vulgare L.) mutants altered in the regulation of synthesis of aspartate-derived amino acids were sought by screening embryos for growth on a medium containing lysine plus threonine. One mutant, Rothamsted 2501, was selected with good growth. From the segregation of resistance in the following generations, it was concluded that the resistance was conferred by a dominant gene, Lt1. No homozygous Lt1/Lt1 fertile plants have been recovered. Partially purified aspartate kinase preparations from resistant and sensitive plants were separated on DEAE-cellulose chromatography into three peaks of activity (I, II, III) and the feedback regulatory properties of these peaks determined. These peaks are considered to be three isozymic forms of aspartate kinase, one predominantly sensitive to threonine and two sensitive to lysine or lysine plus S-adenosyl methionine. The feedback characteristics of one of the peaks of aspartate kinase activity from resistant plants were changed such that lysine was half-maximally inhibitory at 10 rather than 0.4mm. Increases in the concentrations of the free pools of threonine (4×) and methionine (2×) were measured in young plants grown on a basal medium. Threonine in the soluble fraction of mature seeds from resistant plants was increased from 0.8 to 9.6% of the total threonine content. The total content of both threonine and methionine of the seeds was increased by 6% compared with grain of similar nitrogen content.
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