ISSN:
1617-4623
Keywords:
Plasmid R1
;
Stability functions
;
ParD system
;
Cell killing functions
;
kis and kid loci
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The proteins P10 and P12 have been shown to be gene products of a new stability system, ParD, of plasmid R1. It is now shown that an R1 miniplasmid, pAB112, carrying a trans-complementable amber mutation in the gene of the P10 protein, is lethal for the host in the absence of suppression. This lethal effect is suppressed in a supF background and also by deletions in pAB112 that affect the gene of the P12 protein. These data indicate that the P12 protein has a lethal effect on the host and that this effect is neutralized by the P10 protein. The possibility that the stabilization conferred by the ParD system could be due to a counterselection, mediated by P12, of cells that lose the plasmid at cell division, is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331316
Permalink