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  • 1
    Electronic Resource
    Electronic Resource
    Pulsed Electron Beam Annealing of GaAs after High Dose Implantation of Hydrogen (1997)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 248-249 (May 1997), p. 253-256 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/02/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.248-249.253.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Sterol synthesis in vitro in freshly isolated blood mononuclear leukocytes from familial hypercholesterolemic patients treated with probucol
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 833 (1985), S. 412-416 
    Details
    ISSN: 0005-2760
    Keywords: (Human mononuclear leukocytes) ; Cholesterolemia ; Cholestyramine ; Plasma cholesterol ; Probucol ; Sterol synthesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2760(85)90098-0
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  • 3
    Electronic Resource
    Electronic Resource
    β-1,3-Glucanases in wheat and resistance to the Russian wheat aphid (1998)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 103 (1998), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko) is one of the most destructive insect pests of cereals world-wide. Although resistant cultivars have been bred, the biochemical mechanism of resistance is unknown. The aim of this work was to gain information on the mechanism of resistance which could contribute to more directed breeding of resistant cultivars in the future. The effect of RWA infestation on the inter- and intracellular β-1,3-glucanase activities was studied in different resistant wheat (Triticum aestivum L.) cultivars containing the Dn-1 gene for RWA resistance and corresponding near-isogenic susceptible cultivars. The activity was determined spectrophotometrically by measuring the release of glucose from laminarin. Infestation differentially induced the intra- and intercellular activities to much higher levels in resistant than susceptible cultivars within 48 h. According to immunological studies induced enzyme activities were due to increased protein levels. The intracellular β-1,3-glucanase contained about 8% exo-activity. The exo-activity made an insignificant contribution to the intercellular activity. The genetic background into which the resistance gene was bred did affect the level of activity that corresponded to the resistance performance. Seven apoplastic isoforms of β-1,3-glucanase, varying from acidic to basic, were resolved by isoelectric focusing. All isoenzymes were equally induced and no specific one could be linked to resistance or susceptibility. The RWA induced β-1,3-glucanase activity in resistant cultivars closely resembles defence responses during pathogenesis and seems to be part of a general defence response like the hypersensitive reaction (HR), which confers resistance to the RWA. This knowledge might be helpful in future to identify genes for RWA resistance. The increased β-1,3-glucanase activity after RWA infestation might serve as an additional measure to biochemically trace resistance in crosses during breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.1998.1030115.x
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic effects and heterosis of flowering and fruit characteristics of tropical pumpkin (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Tropical pumpkin is an important vegetable in many lowland tropics. Estimation of genetic effects for a fixed set of genotypes may yield information on the presence of different types of gene action for important traits. Six landraces and one diallel set of their progeny were evaluated for time from planting to female anthesis and fruit characteristics. Variety effects and heterosis mean squares were significant for the days to female anthesis, the weight of the first mature fruit, the mean fruit weight and soluble solids, indicating the presence of both additive and non-additive gene actions. Only variety effects were significant for the days to the first mature fruit. These characters could be improved by recurrent selection. Selection for heterosis would be effective except for the days to the first fruit to mature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00595.x
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  • 5
    Electronic Resource
    Electronic Resource
    Mucor—a source of cocoa butter and gamma-linolenic acid (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 417-422 
    Details
    ISSN: 1573-0972
    Keywords: Acetic acid ; cocoa butter ; gamma-linolenic acid ; lipid ; Mucor ; oil ; triacylglycerol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Lipid analyses were performed on 28 strains of various species of the genus Mucor. In shake flasks with glucose as carbon source, the gamma-linolenic acid (GLA) content in the neutral lipid (NL) fraction of some Mucor species was up to 38 mg GLA/g dry biomass. Some Mucor species produced more than 20% (w/w) stearic acid (18:0) and more than 60% of their NL content as symmetrical triacylglycerols (SUS-TAGs) which corresponded to those of cocoa butter. Three Mucor species were evaluated in terms of the production of SUS-TAGs and GLA in pH-stat, fed-batch cultures in an air-lift fermenter with acetic acid as titrant and carbon source. Mucor circinelloides f. circinelloides CBS 108.16 accumulated 27% 18:0 in the NL fraction, which constituted approximately 40% of the dry biomass. In this case, the NL fraction contained more than 70% (w/w) SUS-TAGs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00144464
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  • 6
    Electronic Resource
    Electronic Resource
    Enhanced sunflower oil utilization and gamma-linolenic acid production by Mucor circinelloides f.circinelloides CBS 108.16 in the presence of acetate (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 357-358 
    Details
    ISSN: 1573-0972
    Keywords: Acetate ; gamma-linolenic acid ; Mucor circinelloides ; sunflower oil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018507712965
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  • 7
    Electronic Resource
    Electronic Resource
    Hydroxy long-chain fatty acids in fungi (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 495-504 
    Details
    ISSN: 1573-0972
    Keywords: Cyclooxygenase ; cytochrome P-450 monooxygenase ; fungi ; hydroxy long-chain fatty acids ; lipoxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Hydroxy long-chain fatty acids occur widely in animals and plants and have important physiological activities in these eukaryotes. There are indications that these compounds are also common and important in fungi. The occurrence of hydroxy-polyunsaturated fatty acids (hydroxy-PUFAs) is of biotechnological importance, because these compounds are potentially high-value lipid products with medical applications. This review pays particular attention to the production of hydroxy-PUFAs by yeasts and other fungi. Hydroxy-PUFAs derived from lipoxygenase activity appear to be present in most fungi, while hydroxy-PUFAs from cyclooxygenase activity (i.e. prostaglandins) have mainly been implicated in the Oomycota and in yeasts from the genus Dipodascopsis. The occurrence of other hydroxy long-chain fatty acids in fungi is also discussed briefly; these include hydroxy fatty acids that are generally associated with cytochrome P-450 monooxygenase activity (i.e. terminal and sub-terminal hydroxy acids and diols derived from the corresponding epoxides) as well as 2-hydroxy-fatty acids and 3-hydroxy-fatty acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367653
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  • 8
    Electronic Resource
    Electronic Resource
    Differential induction of apoplastic peroxidase and chitinase activities in susceptible and resistant wheat cultivars by Russian wheat aphid infestation (1998)
    Springer
    Plant cell reports 18 (1998), S. 132-137 
    Details
    ISSN: 1432-203X
    Keywords: Key words Russian wheat aphid resistance ; Apoplast ; Chitinase ; Peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intercellular peroxidase and chitinase activities of three wheat cultivars [Triticum aestivum L. cvs `Tugela DN', `Molopo DN' (Gariep) and `Betta DN'] containing the Dn-1 gene for resistance to the Russian wheat aphid (RWA) Diuraphis noxia (Mordvilko) and the corresponding near-isogenic susceptible cultivars (`Tugela', `Molopo' and `Betta') were studied under conditions of infestation and non-infestation. The aim was to gain information on the mechanism of resistance. The resistance response was induced by RWA infestation. Infestation rapidly induced the activities of both enzymes selectively in resistant wheat to levels of magnitudes higher than those in susceptible wheat. The genetic background in which the Dn-1 resistance gene is bred played a role and the level of activity corresponded to the level of resistance. Immunologic studies confirmed that the induction of enzyme activities was due to the induction of higher protein levels. These results indicate that peroxidase and chitinase may have a role in insect resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050545
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  • 9
    Electronic Resource
    Electronic Resource
    Development of RAPD and SCAR markers linked to the Russian wheat aphid resistance gene Dn2 in wheat (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1162-1169 
    Details
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Russian wheat aphid ; Dn2 resistance gene ; RAPD ; SCAR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  RAPD (random amplified polymorphic DNA) analysis was used to identify molecular markers linked to the Dn2 gene conferring resistance to the Russian wheat aphid (Diuraphis noxia Mordvilko). A set of near-isogenic lines (NILs) was screened with 300 RAPD primers for polymorphisms linked to the Dn2 gene. A total of 2700 RAPD loci were screened for linkage to the resistance locus. Four polymorphic RAPD fragments, two in coupling phase and two in repulsion phase, were identified as putative RAPD markers for the Dn2 gene. Segregation analysis of these markers in an F2 population segregating for the resistance gene revealed that all four markers were closely linked to the Dn2 locus. Linkage distances ranged from 3.3 cM to 4.4 cM. Southern analysis of the RAPD products using the cloned RAPD markers as probes confirmed the homology of the RAPD amplification products. The coupling-phase marker OPB10880c and the repulsion-phase marker OPN1400r were converted to sequence characterized amplified region (SCAR) markers. SCAR analysis of the F2 population and other resistant and susceptible South African wheat cultivars corroborated the observed linkage of the RAPD markers to the Dn2 resistance locus. These markers will be useful for marker-assisted selection of the Dn2 gene for resistance breeding and gene pyramiding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050852
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  • 10
    Electronic Resource
    Electronic Resource
    Development of markers linked to Diuraphisnoxia resistance in wheat using a novel PCR-RFLP approach (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 965-970 
    Details
    ISSN: 1432-2242
    Keywords: Key words Russian wheat aphid ; Near-isogenic lines ; Restriction digests ; RAPD ; SCAR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstracts  Through random amplified polymorphic DNA (RAPD) analysis we identified a putative marker linked to the Dn5 resistance gene. This marker was converted to a more reliable sequence-characterised-amplified regions (SCAR) marker. The initial SCAR marker amplified the correct amplification product but failed to discern between the susceptible and resistant individuals. Hence, it was utilised to sequence the internal fragment. All nested primers designed from the internal sequences were also unable to produce any polymorphism between the susceptible and resistant cultivars. Restriction digests were then performed on these fragments, and the restriction enzyme EcoRI was able to discern between the susceptible and resistant F2 individuals of the Dn5 population. This granted one marker amplified with the internal SCAR primer set OPF141083 the ability to differentiate between parental individuals carrying the Dn5 genes. This marker was tested in a segregating F2 population carrying the Dn5 resistance gene and proved able to differentiate between the segregating individuals. This marker may prove useful in marker assisted selection (MAS), although performing restriction digests may hamper the throughput of a high number of samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051377
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