ISSN:
1432-136X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Ion transport processes in the ileum of the lizard,Gallotia (=Lacerta) galloti was examined in vitro by measuring Na22 and Cl36 fluxes across short-circuited preparations. In Ringer-bicarbonate solution there was both a net sodium flux ( $$J_{{\text{net}}}^{{\text{Na}}^{\text{ + }} } $$ ) and a net chloride flux ( $$J_{{\text{net}}}^{{\text{Cl}}^ - } $$ ) from mucosa to serosa. The inequality between $$J_{{\text{net}}}^{{\text{Na}}^{\text{ + }} } $$ and short-circuit current (I sc) suggests that part of the net sodium transport is the result of an electrically neutral transport mechanism or that another electrogenic mechanism opposite in sign is contributing to the short-circuit current. In the absence of sodium, the short-circuit current and net chloride flux were abolished. In the absence of chloride, the net sodium was reduced but not abolished and the short-circuit current was unchanged. From an analysis of the effects of the inhibitors furosemide, amiloride, disulfonic stilbene (DIDS) and acetazolamide, a plausible model was developed to explain the characteristics of these transports. It is proposed that the entry of sodium into the cell across the luminal membrane occurs by two pathways. Part occurs by the antiport Na+H+ and part by an electrogenic pathway. The entry of chloride is by the antiport Cl−HCO 3 − .
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00691019
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