ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Feruloyl esterases cleave ferulic acid from arabinoxylan and pectin. Feruloyl groups are believed to crosslink the polysaccharide chain within the polymer and to link hemicellulose to lignin, which may play a role in controlling the growth of plants. The Clostridium thermocellum cellulosome xylanase Z feruloyl esterase was expressed in Escherichia coli, purified and crystallized. The crystals diffract to 2.4 Å resolution and belong to the orthorhombic space group P212121, with unit-cell parameters a = 43.14, b = 63.77, c = 79.57 Å. Assuming one molecule per asymmetric unit, the Matthews coefficient is calculated to be 1.87 Å3 Da−1, which corresponds to a solvent content of 34%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900006521
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