ISSN:
1432-203X
Keywords:
Abbreviations: CM, Coconut milk; 2,4-D, 2,4-dichlorophonoxyacetic acid; GA3 , gibberellic acid; MS, Murashige and Skoog (1962) medium; NAA, naphthaleneacetic acid; TDZ, thidiazuron
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. Pepper (cv. New Mexico – 6 and Rajur Hirapur) plants were regenerated from immature zygotic embryos via direct somatic embryogenesis. Somatic embryos were formed directly, without any intervening callus, on the zygotic embryo apex, embryo axis and cotyledons on Murashige and Skoog's (MS) medium containing 2,4-D (4-18 μM), thidiazuron (10 μM) and a high concentration of sucrose (6–10%). The best response was observed on MS medium containing 2,4-D (9 μM), coconut water (10%) and high sucrose (8%). The entire process of induction and maturation of the embryos was completed on the same medium. Histological examination indicated that secondary embryogenesis also occurred directly from the primary somatic embryos. Differentiation of embryos was nonsynchronous, and some embryos were swollen and distorted with fasciation. More than 70% of the mature normal somatic embryos germinated readily on MS medium containing GA3 or TDZ, alone and in combination, and following transfer to pots developed into normal plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050069
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