ISSN:
0025-116X
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The homologous oligodeoxyribouridylic acids dU2, dU4, dU8 and dU16 were chemically synthesized in amounts of 70 to 800 mg by block condensation using the phosphotriester method. The partially protected hydroxyl and phosphate components were condensed without any protection for the nucleobase uracil, using a mixture of 2,4,6-triisopropylbenzenesulfonyl chloride and tetrazole in anhydrous pyridine as condensing agent. Demonstrable side reactions of the uracil residue occur under this condensation conditions. However, this undesired derivatisation of uracil residues is totally eliminated by a simple deamination of the deblocked oligodeoxyribouridylic acids. After synthesis, cleavage of the protecting groups and column chromatographical fractionation using QAE-Sephadex, the oligodeoxyribouridylic acids were deaminated and obtained as pure products, as was positively evidenced by “fingerprints”.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/macp.1986.021870412
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