ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Methanobacterium thermoautotrophicum was grown on a defined mineral salts medium under strictly anaerobic conditions with H2 and CO2 as the sole energy and carbon sources, respectively. The cultivation medium was optimized with respect to non-organic components including Se(IV), W(VI), N, Ni(II), Fe(II), Co(II) and Mo(VI). Sulphide concentration in the medium was maintained constant using an on-line regulatory system by the addition of 0.5 M Na2S. A maximum supply rate of 0.6 vvm of a mixture of 80% H2 and 20% CO2 was achieved for the gaseous substrates. Under these conditions a specific growth rate of 0.30 h−1 and a cell concentration of 4.8 g cell dry weight (DW) l−1, representing a 140% increase over previously published results, were obtained. The growth yield of 2.3 g DW mol−1 CH4 was similar to published values. However, the overall specific productivity was enhanced from 11 mmol CH4 g−1 DW h−1 to 24 mmol CH4 g−1 DW h−1, corresponding to an improvement of 120%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00170406
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