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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European biophysics journal 26 (1997), S. 283-290 
    ISSN: 1432-1017
    Keywords: Key words AFM ; Lateral forces ; Nucleo-pore filter ; Immobilization ; Nematocysts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Lateral forces are inevitable in contact mode AFM imaging and they contribute significantly to the image formation under certain conditions. In cases where the objects are comparable in size to the cantilever tip and particularly in cases where the tips have a high aspect ratio, the lateral force may exceed the vertical force and may impose a severe limitation to the stability of the sample during imaging. Here we have calculated the relation between the exerted lateral force and the applied vertical force as a function of the friction coefficient, the geometry of the tip, and the stiffness of the cantilever. We present a strategy to immobilize larger particles by sucking them into the pores of nucleopore filters and binding them by chemical cross linking. High resolution images of nematocysts which were immobilized with this strategy are presented. The images reveal the supra-molecular arrangement of the mini-collagen of the capsule wall.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physica A: Statistical Mechanics and its Applications 207 (1994), S. 500-516 
    ISSN: 0378-4371
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Post-translational histone modification has a fundamental role in chromatin biology and is proposed to constitute a ‘histone code’ in epigenetic regulation. Differential methylation of histone H3 and H4 lysyl residues regulates processes including heterochromatin formation, ...
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    European journal of soil science 53 (2002), S. 0 
    ISSN: 1365-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Except where nitrate is added to the soil artificially, nitrate is leached from forest soils only if it is produced. Although the factors influencing nitrification have been widely studied, nitrification activity still cannot be simply predicted from ecosystem characteristics. In France, about half of the present forest area was agricultural in 1850. Previous work suggested that former cultivation could be a major factor influencing nitrogen availability in forest soils.Using laboratory incubations, we compared the net production of ammonium and nitrate in soils from formerly manured lands planted with conifers 70–100 years ago with that in soils of surrounding ancient coniferous forests. Net nitrate production, available P content, and natural abundance of nitrogen 15, δ15N, were greater in soils from formerly manured plots than other land, whereas the C:N ratio of the soil was less. The difference in net nitrate production between previously manured sites and adjacent ancient forests was related to differences in δ15N values in the soil but not evidently to other soil properties. Because soil δ15N increases with the intensity of organic manuring, these results suggest that nitrification in forest soils depends on previous manurial practices under agriculture. In this context, the soil δ15N might be used as an indicator of both previous agricultural land use and potential nitrification. Because a significant proportion of West European forests grow on previously cultivated soils, past land use should be taken into account when evaluating the risks of nitrate leaching from forests.
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Pesticide Biochemistry and Physiology 26 (1986), S. 284-291 
    ISSN: 0048-3575
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1939
    Keywords: Key words δ15N ; Forest ; Past land use ; N cycling ; Dryopteris carthusiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Since the middle of the 19th century, the area covered by forests in France has doubled. These new forests grow on previous agricultural lands. We have studied the influence of this agricultural history on the 15N abundance of present-day forests planted on farmlands in the Vosges mountains (north-eastern France) between 1898 and 1930. Different types of land use were identified from old cadastres (1814–1836) of 16 farms. Ancient forests adjacent to farmlands were used as controls. Former pastures, meadows, croplands, gardens and ancient forests were compared for soil δ15N (fraction 〈50 µm and total soil), C/N, P and N content and fern (Dryopteris carthusiana) δ15N. The mean δ15N of soil increased in the order ancient forests (+0.0‰)〈pastures (+1.4‰)〈croplands (+1.6‰)〈meadows (+2.5‰)〈gardens (+3.8‰). This increase in soil δ15N with the intensity of former land use was related to the former input of 15N-enriched manure, and to an activation of soil nitrification leading to 15N-depleted nitrate export on previously manured parcels. Fern δ15N increased in the same order as soil δ15N in relation to past land use. The mean δ15N of fern in ancient forests (–4.4‰) and former pastures (–3.4‰) was 5‰ lower than soil δ15N and the two variables were strongly correlated. The δ15N of fern in formerly manured parcels varied little (cropland: –2.7‰, meadows: –2.6‰ and gardens: –2.2‰) and independently of soil δ15N, suggesting that the soil sources of fern N differed between unmanured and manured parcels. Understorey plant δ15N and soil δ15N appear to be excellent tracers of previous land use in forests, and could be used in historical studies. The persistence of high isotopic ratios in previously manured parcels, almost a century after afforestation, suggests a long-term influence of former land use on the N cycle in forest soils.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 13 (2000), S. 631-636 
    ISSN: 1434-6036
    Keywords: PACS. 61.43.Fs Glasses - 71.15.Pd Molecular dynamics calculations (Car-Parrinello) and other numerical simulations - 61.43.Bn Structural modeling: serial-addition models, computer simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: A Car-Parrinello (CP) molecular dynamics simulation of vitreous silica, combined with classical molecular dynamics, is presented. The equilibration of the liquid, quench and relaxation of the glass are performed classically using the van Beest, Kramer and van Santen (BKS) potential [12] and the resulting configuration (coordinates and velocities) is used as input for the CP simulation. A remarkable stability of the CP dynamics is observed justifying this procedure and validating the BKS potential. Several structural and electronic properties are calculated and compared with experiments.
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  • 8
    ISSN: 1573-8248
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The capacity ofTrichogramma evanescens to choice and to exploit the eggs and batch ofOstrinia nubilalis andEphestia kühniella thoroughly the embryonnary development of these host was examinated. A decided choice for theOstrinia batches, one to four days old, was demonstrate by the females distribution on the eggs and by the number of parasited batches. The total parasited eggs was similar in the 2 hosts. T. evanescens prefers vitellous eggs inOstrinia and the germ band age inEphestia. It is able to eliminate the host till the young caterpillar is hatching and can be endo-or ecto-parasite.
    Notes: Résumé La capacité deTrichogramma evanescens Westw. de choisir et d'exploiter es œufs et pontes d'Ostrinia nubilalis Hb et d'Ephestia kuehniella Z. au cours du développement embryonnaire de ces hôtes a été examinée. Un choix marqué vis-à-vis des pontes d'Ostrinia âgées de 1 à 4 jours est démontré tant par la distribution des femelles sur les œufs que par le nombre d'ooplaques parasitées. Le nombre d'œufs total parasités est par contre comparable chez les 2 hôtes.T. evanescens préfère les œufs vitellins chezOstrinia et les œufs au stade bandelette embryonnaire chezEphestia. Il est capable d'éliminer l'hôte jusqu'au stade jeune chenille en train d'éclore et de l'exploiter soit en ecto soit en endo parasite.
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  • 9
    ISSN: 1432-136X
    Keywords: Phosphofructokinase ; Protein kinases ; Glycolyse ; Gill ; Crab, Eriocheir sinensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Phosphofructokinase from the posterior gills of the euryhaline crab Eriocheir sinensis acclimated to freshwater is likely regulated in part via phosphorylation induced by endogenous cyclic nucleotide-dependent protein kinases. Phosphofructokinase from gill extracts devoid of low molecular weight compounds by chromatography through a PD10 Sephadex column, incubated in the presence of cAMP or cGMP protein kinases activators (cAMP or cGMP, Mg-ATP and Mg2+), shows an increased catalytic activity. This treatment is accompanied by 32P incorporation into the proteins immunoprecipitated with anti-mammalian phosphofructokinase polyclonal antibodies cross-reacting with the analog crustacean enzyme. Our results indicate that the covalent modification induced by these nucleotide-dependent protein kinases activates the glycolytic enzyme by increasing its affinity for its substrate and, when the activation is specifically due to cAMP-dependent protein kinases, by also reducing the homotropic cooperativity between its multiple substrate binding sites.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 2003-09-15
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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