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  • 1
    Electronic Resource
    Electronic Resource
    Size of acetylcholine receptors in the membrane. An improved version of the radiation inactivation method (1982)
    s.l. : American Chemical Society
    Biochemistry 21 (1982), S. 2210-2217 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00538a033
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  • 2
    Electronic Resource
    Electronic Resource
    The ultrastructural localization and quantitation of cholinergic receptors at the mouse motor endplate (1973)
    Springer
    The journal of membrane biology 14 (1973), S. 383-402 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The snake venom toxin, α-bungarotoxin, is known to bind specifically to the acetylcholine receptor at skeletal muscle endplates. In this study, tritiated α-bungarotoxin has been used in conjunction with electron-microscope autoradiography to visualize and enumerate acetylcholine receptor sites at the neuromuscular junctions of the mouse diaphragm. From an analysis of the grain distribution, the receptor sites appear to be located specifically on the postjunctional membrane. The density there is about 8,500/μ2 of membrane surface. For comparison purposes, cholinesterases and related active centers were labeled using [3H] diisopropylfluorophosphate; they were shown to be at this same concentration over the synaptic membranes (or along the cleft). The 1∶1 relationship of the receptors to the cholinesterase type of site, found previously to hold in studies on whole endplates, is also true at the ultrastructural level in this case. In fact, this 1∶1 relationship is believed to be a characteristic of the postsynaptic membranes of endplates in other muscles and other vertebrates. Based on the constant density value thus arrived at, the total surface areas of postsynaptic and of presynaptic membranes are at once obtained from the known total numbers of these sites per endplate, available from previous studies in this laboratory. Examples of such synaptic surface area values are given. These values are only reliable for a given muscle type if the approximate fiber size is defined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868086
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  • 3
    Electronic Resource
    Electronic Resource
    The density of cholinergic receptors at the endplate postsynaptic membrane: Ultrastructural studies in two mammalian species (1975)
    Springer
    The journal of membrane biology 20 (1975), S. 31-49 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Electron-microscope autoradiography of diaphragm endplates of the American brown bat, labeled to saturation with tritiated α-bungarotoxin, has been used as a means to localize and quantitate the acetylcholine receptor there. Analysis of the grain distribution in these autoradiographs reveals that the receptor sites in this endplate are located on the postsynaptic membrane at an average density of 8,800/μ2. The sites are distributed asymmetrically along that membrane, being concentrated at the crests of the postjunctional folds—that portion nearest to the presynaptic membrane. The receptor site density at these regions of the postsynaptic membrane is estimated to be 20,000–25,000/μ2 of membrane surface. A comparison of these membrane site densities with those of endplates of red and white fibers of the mouse reveals a close similarity. On this basis, it is suggested that the receptor site density at the crests of the folds may be a characteristic feature of endplates of vertebrates. In contrast to the acetylcholine receptor sites, cholinesterase sites (determined autoradiographically in3H-diisopropylfluorophosphate-labeled endplates) are largely distributed in a uniform manner over the postjunctional folds. The function of the secondary folds is, therefore, reassessed. Ultrastructural evidence available from other laboratories on the spatial characteristics of transmitter release and of postsynaptic dense particles is in accord with a model drawn for this molecular architecture at the vertebrate endplate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01870626
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  • 4
    Electronic Resource
    Electronic Resource
    A Cytochemical Reaction for Nucleoprotein (1956)
    [s.l.] : Nature Publishing Group
    Nature 178 (1956), S. 1450-1453 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] WE have recently completed a study of a cytochemical reaction for protein which appears to be specific for protein in a special form of combination with nucleic acid. When a diazonium hydroxide is applied to a tissue section, the following components may react: (1) tyrosine and histidine and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1781450a0
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  • 5
    Electronic Resource
    Electronic Resource
    Quantitative Cytochemical Observations on a Specific Nucleoprotein Reaction in Cell Nuclei (1960)
    [s.l.] : Nature Publishing Group
    Nature 186 (1960), S. 447-449 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] AN application of micro-spectrophotometry to a specific protein cytochemical reaction is reported here, forming part of an attempt to understand the significance of a particular bonding observed in nucleoprotein in cell nuclei. It has been shown1 that a particular component of the nucleoprotein of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/186447a0
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  • 6
    Electronic Resource
    Electronic Resource
    Selective Cytochemical Demonstration of Arginine Groups in Nucleoprotein in the Cell Nucleus (1960)
    [s.l.] : Nature Publishing Group
    Nature 187 (1960), S. 508-509 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The main obstacles to its reliable exploitation for the purpose mentioned are : (i) the contribution of arginine groups not involved in the deoxyribonucleic acid-protein complex ; (2) the stain in surrounding cytoplasm, necessitating the use of isolated nuclei,, with concurrent artefacts ; (3) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/187508a0
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  • 7
    Electronic Resource
    Electronic Resource
    Quantitative Cytochemistry using Directly Applied Radioactive Reagents (1961)
    [s.l.] : Nature Publishing Group
    Nature 189 (1961), S. 412-413 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To establish the basis of the method, the acylating reagent acetic anhydride, labelled with tritium, was applied (0-4 M) in pyridine-acetonitrile as a general blocking agent3, to indicate the total number of groups available to acylation, notably -NH2, -OH and -SH groups in macro-molecules. Frog ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/189412a0
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  • 8
    Electronic Resource
    Electronic Resource
    Ribonucleases (1969)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Biochemistry 38 (1969), S. 677-732 
    Details
    ISSN: 0066-4154
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Chemistry and Pharmacology , Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.bi.38.070169.003333
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  • 9
    Electronic Resource
    Electronic Resource
    Specific Proteases of the Rat Mast Cell: Intracellular Localization of Specific Proteases in Rat Mast Cells (1967)
    [s.l.] : Nature Publishing Group
    Nature 213 (1967), S. 1202-1203 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE possibility of applying radioactive organophosphate inhibitors of high specific activity to localize sites of esterase activity by autoradiography has permitted estimates, in several specific cases, of the number of enzyme active centres present in individual intra-cellular sites1'2. For the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2131202a0
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  • 10
    Electronic Resource
    Electronic Resource
    Specific Derivatives of Ribonuclease for Crystallographic Determination of the Protein Structure (1967)
    [s.l.] : Nature Publishing Group
    Nature 213 (1967), S. 562-563 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE X-ray diffraction studies on bovine pancreatic ribonuclease described in the preceding article required the preparation of several different isomorphous crystals, each of which contained one or a few stable, heavy atoms at defined locations in the crystal. Knowledge of the site of attachment of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/213562a0
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