ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Adenosine in blood
;
Isocratic elution
;
Stop-solution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Due to manifold physiological and cardioprotective actions of adenosine, the demand for a simple but accurate method to determine its concentration in plasma is increasing. The aim of this study was firstly to develop a simple isocratic method instead of the gradient elution or peak-shifting techniques used earlier and secondly to check conflicting data on the composition of “stop-solution”, added to the sample in order to prevent changes in adenosine concentration. Isocratic elution improved signal to noise ratio and concentrations of 100 μmol L−1 dipyridamole and 2.5 μmol L−1 erythro-9(2-hydroxy-3-nonyl)adenine in the blood sample effectively prevented both adenosine formation and degradation, even without the use of a 5′-ecto-nucleotidase inhibitor. Lowering the concentration of dipyridamole to 25 μmol L−1 caused more than a tenfold increase of adenosine concentration in two out of five cases and even 100 μmol L−1 dipyridamole alone is not sufficient to inhibit adenosine deaminase in blood samples.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290317
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