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  • 1
    Publication Date: 2018-01-24
    Description: Pesticides, including herbicides, insecticides and fungicides, are widely used in intensive agriculture. Recently, the long-term effects of pesticide exposure were found to be associated with many diseases. In this study, we evaluated the long-term effect of low-level exposure to a mixture of pesticides on DNA damage response (DDR) in relation to individual detoxifying variability. A residential population chronically exposed to pesticides was enrolled, biological/environmental pesticide levels; paroxonase 1 (PON-1) activity and 192 Q/R polymorphism and DDR were evaluated at three different periods of pesticide exposure. OGG1-dependent DNA repair activity was decreased in relation to pesticide exposure. The increase of DNA lesions and pesticide levels in the intensive pesticide-spraying period was independent on PON-1 activity. Next, human bronchial epithelial and neuronal cells were used as a model for in vitro evaluation of the mechanistic effect of pesticides. Pesticides induced mitochondrial dysfunction leading to ROS formation. ROS from mitochondria induced DNA damage, which in turn induced OGG1-dependent DNA repair activity through 8-oxoguanine DNA glycosylase 1 (OGG1) expression and activation. Even though OGG1 was overexpressed, an inhibition of its activity, associated with DNA lesion accumulation, was found at prolonged pesticide-exposure. A post-translational regulation of OGG1 by pesticide may be postulated. Taken together, long-term exposure to low-levels of pesticides affects DDR resulting in accumulation of DNA lesions that eventually may lead to cancer or neurological disorders.
    Print ISSN: 1520-4081
    Electronic ISSN: 1522-7278
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Wiley
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  • 2
    Publication Date: 2023-03-06
    Description: The Nirano Salse, known since the Roman Times, are one of the most beautiful and scenic mud volcanoes areas of Italy with thousands of visitors every year. In this work, we apply novel (for the context) hydrogeological techniques to characterize mud levels in the Salse by means of GPS-RTK positioning and continuous level logging within mud conduits. This is important to quantify the gas–liquid ratio in the conduits and evaluate the potential for dangerous abrupt mud eruptions. The results presented suggest that different mud levels in mud volcanoes clusters are due to the different gas–liquid ratio in the conduits and not necessarily exclude interconnection at depth, a hypothesis, on the other hand, that seems strengthened by mud level time series correlations. The presence of shallow aquifers at a depth of 5 to 30 m is also supported by our field data and allows us to delineate the boundaries of the shallow mud reservoir—pipes system and its overall shape. The shallow aquifers may provide a temporary storage for the ascending gas and when fluid pressure in these aquifers exceeds the tensional strength of the sedimentary rock, leakage of fluids to the surface would occur. In this case, if the gas–liquid ratio is high, mud volcanoes develop into tall gryphons and tend to have a discontinuous activity with sudden eruptions of mud after long periods of quiescence. This, together with the knowledge of shallow conduits localization has an important implication for site safety in proximity to the mud volcanoes. Our inferences based on mud level relationships to mud extrusion dynamics can be applied to lower risk in other mud volcanoes areas of the world with high geo-tourist visits, such as those of Trinidad, Azerbaijan, and Colombia.
    Description: Published
    Description: 480
    Description: 2TR. Ricostruzione e modellazione della struttura crostale
    Description: JCR Journal
    Keywords: Mud volcanism ; Aquifer ; Mud level ; Mud flow dynamics ; Nirano Salse
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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