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  • 1
    Electronic Resource
    Electronic Resource
    Diatom mineralization of silicic acid (1979)
    Springer
    Archives of microbiology 123 (1979), S. 157-164 
    Details
    ISSN: 1432-072X
    Keywords: Active transport ; Binding ; Cell cycle ; Diatoms ; Germanium-68 ; Ion regulation ; Light dependent transport ; Nitzschia angularis ; Transport regulation ; Silicic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Silicic acid binding, possibly at a membrane site is suggested as the first step in Si(OH)4 metabolism in the marine diatom Nitzschia angularis. We estimate 1738±133 Si(OH)4 binding sites·μm-2 cell surface and a turnover of 4–12 Si(OH)4 molecules·s-1 at maximum transport velocity. All studies were carried out using 68Ge(OH)4 as a tracer for Si(OH)4. After Si(OH)4 uptake, rapid (〈1 min) transformation or intracellular binding was demonstrated; yet the extractable pool size after 1 min was indicative of transport against a concentration gradient. Initial uptake kinetics were linear for 150 s and saturation kinetics were demonstrated with kinetic parameters of 560 pmol Si(OH)4·106 cells-1·min-1 (V max) 4.2 μmol Si(OH)4·L-1(Ks). A metabolic energy requirement for transport was suggested by inhibition of uptake by agents that uncouple or inhibit phosphorylation; transport also was sensitive to agents that block sulfhydryl groups. Such characteristics are consistent with Si(OH)4 transport being an active carrier mediated process. Rates of Si(OH)4 transport were regulated during various growth stages and during the synchronized cell cycle. In Si(OH)4 starved cells, blocked at the initiation of silica frustule formation, cycloheximide treatment caused a rapid decline of transport rate. Blocked cells, placed in the dark, maintained high transport rates for 6 h after which there was a loss of activity during the following 18 h. Light dependent recovery of transport ability in 12 h predarkened cells was dependent on de novo protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446815
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The Role of Lys 394 in Microtubule Assembly (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 466 (1986), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1986.tb38425.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of exogenous serotonin on a motor behavior and shelter competition in juvenile lobsters (Homarus americanus) (2000)
    Springer
    Journal of comparative physiology 186 (2000), S. 575-582 
    Details
    ISSN: 1432-1351
    Keywords: Key words Serotonin ; 5-Hydroxytryptamine ; Motor behavior ; Shelter competition ; Aggression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Three experiments were conducted to determine (1) the pharmacodynamics of 5-hydroxytryptamine in juvenile lobsters; (2) the effects of 5-hydroxytryptamine, using a range of dosages, on a motor behavior used to escape an aversive situation; and (3) the effect of doses that did and did not inhibit this motor behavior on measures of dominance and shelter competition. The fate of 5-hydroxytryptamine in hemolymph over a 60-min post-injection period showed that the concentration fell rapidly to a low plateau that was maintained for at least 1 h. Low doses of 5-hydroxytryptamine did not affect locomotor behavior, but higher doses inhibited it. Dominance and subsequent possession of a shelter were unaffected by a low dose of 5-hydroxytryptamine but a higher dose that inhibited locomotion resulted in lobsters that lost fights and did not secure or retain possession of the shelter. In the context of dominance and shelter competition, we were unable to demonstrate any advantage of the low dose of exogenous 5-hydroxytryptamine and a severe disadvantage with the higher dose. Previous reports of transient increases in aggression in 5-hydroxytryptamine-treated subordinate lobsters did not take into account motor inhibition as a possible critical variable in aggression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003590000113
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  • 4
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    Diatom mineralization of silicic acid (1979)
    Springer
    Details
    Publication Date: 1979-11-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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