ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
As a prelude to an eventual pilot plant scale-up of the collagen-β-galaotosidase system, mass transfer, kinetic, pH and temperature profiles were completed. The diffusional studies proved useful in elucidating the role of internal and external mass transfer in the collagen system. External mass transfer was negligible for a residence time in the range of 41.2 to 4.1 × 10−3 min or a flow rate of 48–495 ml/min. Internal transport influence increased with increasing film thickness. A thin film (0.4 mil or 10.16 × 10−3 mm) produced the highest activity per gram of complex. It is not advisable to use a film thinner than 0.4 mil with a bench-top reactor since the film will lack sufficient mechanical strength. Separate pH and temperature profiles were obtained with respective optima being 3.5 and 55°C. An activation energy of 10.7 kcal/mole was calculated by means of an Arrhenius plot. A kinetic study showed that the Michaelis constants for both the immobilized and soluble β-galactosidase were similar, 0.066 and 0.060M, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1976.tb00654.x
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