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  • 1
    Electronic Resource
    Electronic Resource
    Transcription and processing of ribonucleic acid in Rhynchosciara salivary glands. II. Hybridization of nuclear and cytoplasmic ribonucleic acid with nuclear deoxyribonucleic acid. Indication of deoxyribonucleic acid amplification (1972)
    s.l. : American Chemical Society
    Biochemistry 11 (1972), S. 3672-3680 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00770a002
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  • 2
    Electronic Resource
    Electronic Resource
    Transcription and processing of ribonucleic acid in Rhynchosciara salivary glands. I. Rapidly labeled ribonucleic acid (1972)
    s.l. : American Chemical Society
    Biochemistry 11 (1972), S. 3663-3672 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00770a001
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  • 3
    Electronic Resource
    Electronic Resource
    Serum and hormonal regulation of the “resting-proliferative” transition in a variant of 3T3 mouse cells (1977)
    [s.l.] : Nature Publishing Group
    Nature 265 (1977), S. 148-151 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Effect of hydrocortisone (H), pituitary factor (PF) and calf serum (S on 3H-thymidine uptake (0.25 µC ml-1,10 -7 M) into DNA for three different clones of Swiss 3T3 cells (American Type Culture Collectio1). Protocol: Starvation Restimulation DNA synthesis assay ? Abscissa shows ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/265148a0
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  • 4
    Electronic Resource
    Electronic Resource
    Relevance of c-fos proto-oncogene induction for the steroidogenic response to ACTH, dcAMP and phorbol ester in adrenocortical cells (1993)
    Springer
    Molecular and cellular biochemistry 124 (1993), S. 23-32 
    Details
    ISSN: 1573-4919
    Keywords: ACTH ; phorbol ester ; dibutyryl cAMP ; adrenocortical cells ; steroidogenic response ; c-fos proto-oncogene induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We previously reported that ACTH, but not dibutyryl cAMP, rapidly induces the c-fos proto-oncogene in Y-1 adrenocortical cells. Here we show that PMA induces c-fos with similar kinetics when compared with ACTH (0.5–1 h peak) but reaches only 60% of the maximal ACTH induction and dcAMP is a weak c-fos inducer (15% of ACTH). However, combination of PMA and dcAMP has a synergistic effect leading to maximal c-fos induction. c-fos expression may play a role in the RNA synthesis-dependent corticosteroidogenesis response and/or growth regulation by ACTH. We also show that, in contrast to dcAMP, PMA is a poor steroidogenesis stimulator (15 to 17% of maximum ACTH-stimulated level), its activity being completely dependent on RNA synthesis. Combination of dcAMP and PMA yields an additive steroidogenesis stimulation, an effect that is also dependent on RNA synthesis. Although no strict correlation was found between c-fos induction and early steroidogenesis stimulation, particularly with respect to cAMP derivatives, the results suggest that a PKC pathway is likely to cooperate with the classical cAMP-PKA pathway in adrenal cells' RNA-dependent steroidogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01096378
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  • 5
    Electronic Resource
    Electronic Resource
    Ca2+ and Mg2+ requirements for growth are not concomitantly reduced during cell transformation (1984)
    Springer
    Molecular and cellular biochemistry 59 (1984), S. 173-181 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The effects of Mg2+ and Ca2+ deprivation on survival and growth of normal and transformed cultured cells were studied. Normal fibroblast from several origins (mouse, hamster and human) did not grow in 2 µM Mg2+ medium, whereas transformed fibroblasts (mouse and rat) and tumor cells of other types (mouse adrenocortical and rat glial cells) grew optimal. All transformed or tumorogenic cells that showed low growth requirement for Mg2+ were able to develop colonies in agarose suspension cultures, i.e., were anchorage-independent for growth. Mg2+ deprivation of normal cells did not lead to cell cycle arresting at Go/ Gl and cell death accounts for the limited growth of these cells in medium containing low Mg2+ concentration. Contrary to Mg2+, starvation for serum or for Ca2+ caused normal cells to undergo cell cycle arrest at the Go/Gl phase. During the progressive spontaneous transformation of Swiss mouse 3T3 fibroblasts, the decrease in growth requirements for Mg2+ and Ca2+ do not occur at the same time. The requirement for external Ca2+ lowers before the onset of anchorage-independent growth while the Mg2+ requirement only decreases after cells become anchorage-independent. Therefore the reductions in growth requirement for Mg2+ and Ca2+ that take place in cell transformation are not linked events.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231313
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  • 6
    Electronic Resource
    Electronic Resource
    Glucocorticoid dexamethasone reversibly complements EJ-ras oncogene to transform mouse embryo BALB-3T3 cells (1989)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 41 (1989), S. 171-177 
    Details
    ISSN: 0730-2312
    Keywords: EJ-ras oncogene transfectant ; glucocorticoid hormone ; reversible transformation ; p21ras ; β-IFN ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: EJ-A is a Balb-3T3 transfectant cell line that bears a small number of EJ-ras oncogene copies/cell, has low EJ-ras expression, and resembles the parental cell line in displaying a non-transformed phenotype. The glucocorticoid hormone dexamethasone reversibly induces transformation traits in EJ-A cells, namely: (1) morphological transformation; (2) increased growth rate and saturation density; (3) reduced G1 length; and (4) independence of the FGF requirement to initiate DNA synthesis. Western blot analysis revealed that dexamethasone does not increase the p21ras protein intracellular level. β-IFN, added to the culture medium, does not suppress the dexamethasone-induced growth stimulation and morphological transformation. Therefore, glucocorticoid hormones can complement low EJ-ras expression to transform Balb-3T3 cells, by a mechanism that is likely to be independent of p21ras increase and β-IFN decrease.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240410402
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  • 7
    Electronic Resource
    Electronic Resource
    Stimulation of heparan sulfate proteoglycan synthesis and secretion during G1 phase induced by growth factors and PMA (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 70 (1998), S. 563-572 
    Details
    ISSN: 0730-2312
    Keywords: heparan sulfate and growth factors ; heparan sulfate and phorbol ester ; heparan sulfate and cell cycle ; proteoglycans and cell cycle ; cell cycle; phorbol ester and heparan sulfate ; heparan sulfate and PKC ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Fetal calf serum (FCS) and PMA (phorbol 12-myristate-13-acetate) specifically stimulate the synthesis of heparan sulfate proteoglycan in endothelial cells. Staurosporine and n-butanol, kinase inhibitors, abolish the PMA effect. Forskolin and 8-bromo adenosine 3′:5′-cyclic monophosphate, activators of, respectively, adenylate cyclase and protein kinase A cannot reproduce the PMA effect. The kinetics of cell entry into S phase of the endothelial cells was determined by DNA synthesis ([3H]-thymidine and Br-dU incorporation), and flow cytometry. The mitogenic effect of fetal calf serum is abolished by PMA. Also, PMA pre-treatment inhibits the enhanced synthesis of heparan sulfate proteoglycan after a second PMA exposure. Remarkably, the stimulation of heparan sulfate proteoglycan synthesis by fetal calf serum and PMA seems to be mainly restricted to G1 phase. Therefore fetal calf serum and PMA cause an enhanced synthesis of heparan sulfate proteoglycan, and PMA causes a cell cycle block at G1 phase. J. Cell. Biochem. 70:563-572, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    On the regulation of DNA synthesis in a line of adrenocortical tumor cells: Effect of serum, adrenocorticotropin and pituitary factors (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 93 (1977), S. 1-9 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Y-1 adrenal cells responded to serum step down by a several fold decrease in DNA synthesis. Serum starved cells resumed DNA synthesis upon serum step up. ACTH and cAMP inhibited DNA synthesis both at low and high serum concentrations, a fact previously known. Pituitary, brain and liver crude extracts stimulated DNA synthesis in serum starved cells. Purified pituitary factors preparations contained two activities: one specific for Y-1 cells and another active with both fibroblasts and Y-1 cells. The kinetics of restimulation of DNA synthesis by serum and pituitary factors was studied. DNA synthesis restimulation occurred after a lag of 11 hours. This lag did not vary irrespective of the type of stimulator or its concentration. Cells entered S phase continuously at a rate which increased with increasing concentrations of the stimulator. Cells became refractory to the inhibitory action of ACTH five hours before entering S phase. The implications of these data to the understanding of cell growth control are considered.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040930102
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  • 9
    Electronic Resource
    Electronic Resource
    Glucocorticoid hormone renders rat glioma cells dependent on high concentrations of external Ca2+ for growth (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 115 (1983), S. 99-104 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: C6 rat glioma cells, like other tumor cells, grow optimally in Ca2+- or Mg2+- depleted 5% serum medium. However, the glucocorticoid hormone hydrocortisone renders these cells dependent on high concentrations of external Ca2+ for growth. Upon Ca2+ deprivation (30-80 μM Ca2+ medium) hydrocortisone-treated C6 cells undergo reversible cell-cycle arrest at G0/G1 phase. This effect is specific for glucocorticoid hormones and occurs at physiological concentrations.Growth restimulation of Ca2+-deprived, hydrocortisone-treated C6 cells by bovine pituitary growth factors or serum growth factors only takes place if the external Ca2+ concentration is increased. On the other hand, C6 cell growth requirement for external Mg2+ was not increased by the glucocorticoid hormone treatment. A minimum of 80 μM of external Mg2+ is required to keep cells adhered and spread in Ca2+-depleted (30 μM) 5% serum medium; in high Ca2+ concentration (1.8 mM), Mg2+ is not required for adhesion or spreading. Thus, the hormone hydrocortisone renders the cell cycle of C6 glioma cells controllable by the levels of external Ca2+, a minimal external Mg2+ being necessary to warrant normal adhesion.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041150115
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  • 10
    Electronic Resource
    Electronic Resource
    Anchorage dependence and Ca2+ requirement are independently modulated by hydrocortisone hormone in rat C6 glioma cells (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 117 (1983), S. 155-157 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We had previously shown that hydrocortisone (Hy), a glucocorticoid hormone, regulates the expression of the transformed phenotype of rat C6 glioma cells. The hormone reversibly renders C6 cells dependent on anchorage and high Ca2+ concentration for growth. We had also isolated Hyresistant C6 variants in agarose suspension cultures. Here we report that Hyresistant variants selected in high (1.8mM) Ca2+ medium become growth-arrested in low (30 μM) Ca2+ medium upon hormone treatment. We conclude that Hy-induced anchorage dependence and Hy-induced high Ca2+ requirement for growth of C6 glioma cells, are two independent phenotypes.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041170204
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